Treatment of acute Chlamydia pneumoniae infection with telithromycin in C57BL/6J mice
| Autor: | E. Saario, A. Bryskier, Pekka Saikku, D. Bem David, Liisa Törmäkangas, Maija Leinonen |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Ketolides medicine.medical_specialty medicine.drug_class Antibiotics Telithromycin Pharmacology Biology medicine.disease_cause Mice Pneumonia Bacterial medicine Animals Pharmacology (medical) Lung Ketolide Antibacterial agent Inflammation Chlamydia Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Chlamydia Infections Chlamydophila pneumoniae medicine.disease Antibodies Bacterial Anti-Bacterial Agents Mice Inbred C57BL Infectious Diseases medicine.anatomical_structure Immunoglobulin G Acute Disease Immunology Female Histopathology Macrolides medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 53:1101-1104 |
| ISSN: | 1460-2091 |
| DOI: | 10.1093/jac/dkh213 |
| Popis: | Objectives: The efficacy of telithromycin, a new ketolide antibiotic, was investigated in the treatment of acute Chlamydia pneumoniae infection in a mouse model. Methods: C57BL/6J mice were inoculated intranasally, and the effects of three different doses of telithro- mycin (25, 50 and 100 mg/kg) were assessed after 5 and 10 days of treatment. Lungs for culture, PCR, histo- pathology, and blood for serum samples were collected immediately after each treatment period and at 3 weeks post-inoculation. C. pneumoniae-specific antibodies were analysed, and the effect of treatment was assessed by culture, detection of C. pneumoniae DNA and determination of histopathological inflammatory changes in mouse lungs. Results: Culture negativity in the lungs was achieved with the higher doses, 50 and 100 mg/kg, after 10 days of treatment. C. pneumoniae DNA was not totally eradicated with the treatments, but the groups treated with 50 and 100 mg/kg doses for 10 days had the lowest DNA positivity rates (10%) 3 weeks after the inoculation. In lung histopathology, the efficacy of telithromycin on inflammatory changes was also dose-dependent: higher doses were more effective in reducing the inflammatory reaction. Overall, the 25 mg/kg dose had a weaker effect compared with the others. Conclusions: Telithromycin had both time- and dose-dependent effects on the eradication of chlamydia and on reducing infection-induced inflammatory changes in mouse lungs. |
| Databáze: | OpenAIRE |
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