During capacitation in bull spermatozoa, actin and PLC-ζ undergo dynamic interactions
| Autor: | Salvador Uribe-Carvajal, Itzayana Mejía-Flores, Icela Palma-Lara, Natalia Chiquete-Félix, María de Lourdes Juárez-Mosqueda |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine endocrine system Phospholipase Flagellum 03 medical and health sciences 0302 clinical medicine Perinuclear theca Capacitation Animals Actin 030219 obstetrics & reproductive medicine urogenital system Activator (genetics) Chemistry Acrosome Reaction Cell Biology Actin cytoskeleton Spermatozoa Sperm Actins Cell biology Isoenzymes Actin Cytoskeleton 030104 developmental biology Fertilization Sperm Tail Type C Phospholipases Sperm Motility Cattle Acrosome Sperm Capacitation Protein Binding Developmental Biology |
| Zdroj: | Zygote. 25:558-566 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199417000260 |
| Popis: | SummaryThe migration pattern of sperm-specific phospholipase C-ζ (PLC-ζ) was followed and the role of this migration in actin cytoskeleton dynamics was determined. We investigated whether PLC-ζ exits sperm, opening the possibility that PLC-ζ is the ‘spermatozoidal activator factor’ (SOAF). As capacitation progresses, the highly dynamic actin cytoskeleton bound different proteins to regulate their location and activity. PLC-ζ participation at the start of fertilization was established. In non-capacitated spermatozoa, PLC-ζ is in the perinuclear theca (PT) and in the flagellum, therefore it was decided to determine whether bovine sperm actin interacts with PLC-ζ to direct its relocation as it progresses from non-capacitated (NC) to capacitated (C) and to acrosome-reacted (AR) spermatozoa. PLC-ζ interacted with actin in NC spermatozoa (100%), PLC-ζ levels decreased in C spermatozoa to 32% and in AR spermatozoa to 57% (P< 0.001). The level of actin/PLC-ζ interaction was twice as high in G-actin (P< 0.001) that reflected an increase in affinity. Upon reaching the AR spermatozoa, PLC-ζ was partially released from the cell. It was concluded that actin cytoskeleton dynamics control the migration of PLC-ζ during capacitation and leads to its partial release at AR spermatozoa. It is suggested that liberated PLC-ζ could reach the egg and favour fertilization. |
| Databáze: | OpenAIRE |
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