Monkey erythropoietin gene: cloning, expression and comparison with the human erythropoietin gene
| Autor: | Por-Hsiung Lai, Joan C. Egrie, Chi-Hwei Lin, Sidney Vaughn Suggs, R. Smalling, Kenneth Chen, Fu-Kuen Lin, J K Browne, G. M. Fox, M. Castro |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Transcription
Genetic Biology Kidney Homology (biology) Species Specificity Gene expression Genetics medicine Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Erythropoietin Peptide sequence Gene Base Sequence cDNA library Chinese hamster ovary cell Nucleic Acid Hybridization Kidney metabolism General Medicine Molecular biology Macaca fascicularis Genes Oligodeoxyribonucleotides Female Poly A medicine.drug |
| Zdroj: | Gene. 44:201-209 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(86)90183-6 |
| Popis: | The erythropoietin (Epo) gene from Cynomolgus monkeys has been isolated from a kidney cDNA library using mixed 20-mer oligodeoxynucleotide probes. The gene encodes a 168 amino acid (aa) mature protein with a calculated Mr of 18,490 and a presumptive signal peptide of 24 aa. The Epo gene, when transfected into Chinese hamster ovary (CHO) cells, produces a glycosylated protein with an apparent Mr of 34,000. The expressed product is biologically active in vivo. The monkey gene exhibits 92% and 94% homology to the human gene at the aa and nucleotide sequence levels, respectively. When compared with the human Epo, monkey Epo has an additional 3-aa residue at the N terminus of the mature protein and a deletion of an internal lysine residue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|