An integrated semiconductor device enabling non-optical genome sequencing
| Autor: | William J. Mileski, Rachel Kasinskas, Jonathan Schultz, Mohammad Alanjary, Jacqueline A. Fidanza, Bernard P. Puc, G. Thomas Roth, Annika Branting, Yutao Fu, John H. Leamon, Marina Sedova, Travis A. Clark, Tanya Sokolsky, Michael R. Lyons, Simon Cawley, Wolfgang Hinz, Alan Williams, Matthew D. Edwards, Xin Miao, John F. Davidson, Kevin McKernan, Jeremy Hoon, Eugeni Namsaraev, James Bustillo, Jan Fredrik Simons, Todd Rearick, Eileen T. Dimalanta, Isaac B. Stoner, Jonathan M. Rothberg, Erika Feierstein, David Marran, Jeffrey T. Branciforte, Devin Dressman, Mark James Milgrew, John Nobile, David Light, Martin Huber, Nils Homer, Michelle Schorn, Melville Davey, Brian Reed, Kim L. Johnson, Jeffrey Sabina, Jason W. Myers |
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| Rok vydání: | 2011 |
| Předmět: |
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Light Semiconductor device fabrication Hybrid genome assembly Hardware_PERFORMANCEANDRELIABILITY Bacterial genome size Integrated circuit Biology DNA sequencing law.invention law Hardware_INTEGRATEDCIRCUITS Escherichia coli Humans Massively parallel Vibrio Genetics Multidisciplinary business.industry Genome Human Ion semiconductor sequencing Genomics Sequence Analysis DNA Rhodopseudomonas Semiconductors Human genome business Computer hardware Genome Bacterial |
| Zdroj: | Nature. 475(7356) |
| ISSN: | 1476-4687 |
| Popis: | The seminal importance of DNA sequencing to the life sciences, biotechnology and medicine has driven the search for more scalable and lower-cost solutions. Here we describe a DNA sequencing technology in which scalable, low-cost semiconductor manufacturing techniques are used to make an integrated circuit able to directly perform non-optical DNA sequencing of genomes. Sequence data are obtained by directly sensing the ions produced by template-directed DNA polymerase synthesis using all-natural nucleotides on this massively parallel semiconductor-sensing device or ion chip. The ion chip contains ion-sensitive, field-effect transistor-based sensors in perfect register with 1.2 million wells, which provide confinement and allow parallel, simultaneous detection of independent sequencing reactions. Use of the most widely used technology for constructing integrated circuits, the complementary metal-oxide semiconductor (CMOS) process, allows for low-cost, large-scale production and scaling of the device to higher densities and larger array sizes. We show the performance of the system by sequencing three bacterial genomes, its robustness and scalability by producing ion chips with up to 10 times as many sensors and sequencing a human genome. |
| Databáze: | OpenAIRE |
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