Plant cell cultures as heterologous bio-factories for secondary metabolite production
| Autor: | Tong Wu, Youjun Zhang, Alisdair R. Fernie, Sandra M. Kerbler |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Nicotiana tabacum Arabidopsis Cell Culture Techniques Heterologous Review Article Plant Science Secondary metabolite 01 natural sciences Biochemistry 03 medical and health sciences Synthetic biology Genome editing Plant Cells Tobacco medicine Arabidopsis thaliana Molecular Biology 030304 developmental biology 2. Zero hunger 0303 health sciences biology plant cell culture secondary metabolites fungi food and beverages Cell Biology biology.organism_classification Metabolic Engineering Cell culture Synthetic Biology 010606 plant biology & botany Biotechnology medicine.drug |
| Zdroj: | Plant Communications |
| ISSN: | 2590-3462 |
| DOI: | 10.1016/j.xplc.2021.100235 |
| Popis: | Synthetic biology has been developing rapidly in the last decade and is attracting increasing attention from many plant biologists. The production of high-value plant-specific secondary metabolites is, however, limited mostly to microbes. This is potentially problematic because of incorrect post-translational modification of proteins and differences in protein micro-compartmentalization, substrate availability, chaperone availability, product toxicity, and cytochrome p450 reductase enzymes. Unlike other heterologous systems, plant cells may be a promising alternative for the production of high-value metabolites. Several commercial plant suspension cell cultures from different plant species have been used successfully to produce valuable metabolites in a safe, low cost, and environmentally friendly manner. However, few metabolites are currently being biosynthesized using plant platforms, with the exception of the natural pigment anthocyanin. Both Arabidopsis thaliana and Nicotiana tabacum cell cultures can be developed by multiple gene transformations and CRISPR-Cas9 genome editing. Given that the introduction of heterologous biosynthetic pathways into Arabidopsis and N. tabacum is not widely used, the biosynthesis of foreign metabolites is currently limited; however, therein lies great potential. Here, we discuss the exemplary use of plant cell cultures and prospects for using A. thaliana and N. tabacum cell cultures to produce valuable plant-specific metabolites. Both Arabidopsis thaliana and Nicotiana tabacum cell cultures can be used as bio-factories for the biosynthesis of high-value secondary metabolites in a safe, inexpensive, and environmentally friendly manner. This review discusses recent advances and current challenges in the use of model plant cell cultures for secondary metabolite synthesis. |
| Databáze: | OpenAIRE |
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