Controlled Ovarian Stimulation Protocols Alter Endometrial Histomorphology and Gene Expression Profiles
| Autor: | Patrick W. Keller, Mohammed Kanchwala, Chao Xing, Orhan Bukulmez, Bruce R. Carr, Samir N. Babayev, John Wu, R. Ann Word |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_treatment Estrogen receptor Oocyte Retrieval Stimulation Endometrium Clomiphene Andrology 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Ovulation Induction medicine Humans reproductive and urinary physiology 030219 obstetrics & reproductive medicine In vitro fertilisation medicine.diagnostic_test business.industry urogenital system Wnt signaling pathway Obstetrics and Gynecology Fertility Agents Female female genital diseases and pregnancy complications 030104 developmental biology medicine.anatomical_structure Female Original Article SFRP4 business Transcriptome therapeutics Infertility Female hormones hormone substitutes and hormone antagonists Endometrial biopsy |
| Zdroj: | Reprod Sci |
| ISSN: | 1933-7205 |
| Popis: | Although it is well appreciated that ovarian stimulation protocols for in vitro fertilization (IVF) alter endometrial receptivity, the precise cellular mechanisms are not known. To gain insights into potential mechanisms by which different ovarian stimulation protocols alter the endometrium, we compared histologic and gene expression profiles of endometrium from women undergoing conventional ovarian stimulation for IVF (C-IVF) with those undergoing minimal stimulation with clomiphene citrate (MS-IVF). Sixteen women undergoing MS-IVF (n = 8) or C-IVF (n = 8) were recruited for endometrial biopsy at the time of oocyte retrieval. Endometrial glands were large, tortuous, and secretory with C-IVF but small and undifferentiated with MS-IVF. Whereas RNA sequencing did not reveal changes in estrogen receptor or its co-regulators or classic proliferation associated genes in MS-IVF, together with immunohistochemistry, Wnt signaling was disrupted in endometrium from MS-IVF cycles with significant upregulation of Wnt inhibitors. Secreted frizzled-related protein 1 (sFRP1) was increased fourfold (p |
| Databáze: | OpenAIRE |
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