α-Glucosidase Inhibitory Activity of Tannat Grape Phenolic Extracts in Relation to Their Ripening Stages

Autor: Gaëlle Coussot, Nawel Benbouguerra, Tristan Richard, Cédric Saucier, Ruth Hornedo-Ortega, Josep Valls-Fonayet, Auriane Dudoit
Přispěvatelé: Sciences Pour l'Oenologie (SPO), Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences de la Vigne et du Vin [Villenave d'Ornon] (ISVV), Université de Bordeaux (UB), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), government of Algeria (Ministere algerien de l'Enseignement Superieur et de la Recherche Scientifique), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
030309 nutrition & dietetics
lcsh:QR1-502
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
phenolic compounds
Biochemistry
Article
Antioxidants
Mass Spectrometry
lcsh:Microbiology
Veraison
Anthocyanins
03 medical and health sciences
chemistry.chemical_compound
0404 agricultural biotechnology
Functional food
Stilbenes
Glycoside Hydrolase Inhibitors
Vitis
Gallic acid
Food science
anti-diabetes
Molecular Biology
α glucosidase inhibitory
Chromatography
High Pressure Liquid
Flavonoids
chemistry.chemical_classification
0303 health sciences
Molecular Structure
Plant Extracts
fungi
Polyphenols
food and beverages
alpha-Glucosidases
Ripening
04 agricultural and veterinary sciences
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
grape
040401 food science
ripening
Kinetics
Enzyme
chemistry
Resveratrol
Polyphenol
Anthocyanin
Biocatalysis
α-glucosidase
Zdroj: Biomolecules
Volume 10
Issue 8
Biomolecules, Vol 10, Iss 1088, p 1088 (2020)
Biomolecules, MDPI, 2020, 10 (8), pp.1088. ⟨10.3390/biom10081088⟩
ISSN: 2218-273X
DOI: 10.3390/biom10081088
Popis: The present study aimed to screen grape extracts as novel &alpha
glucosidase inhibitors to prevent type-2 diabetes and hyperglycemia. The total polyphenol content (TPC) was measured by Folin-Ciocalteu assay and the stilbene, anthocyanin and flavan-3-ol compounds were measured by Ultra High-Performance Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS). The &alpha
glucosidase inhibitory of seed and skin Tannat grape extracts at four ripening stages were investigated. The highest TPC values were measured in seeds at the &ldquo
veraison stage&rdquo
(65.29 ±
5.33 g of Gallic Acid Equivalent (GAE) per kilogram of Fresh Weight (FW)). This was in accordance with the high flavan-3-ol contents measured for these two extracts (43.22 ±
2.59 and 45.45 ±
6.48 g/kg of seeds FW, respectively). The skin and seed extracts at the first stage of ripening exerted strong &alpha
glucosidase inhibition, exceeding 95% (p <
0.05). A high linear correlation (R = 0.723, p &le
0.05) was observed between flavan-3-ol contents and the &alpha
glucosidase inhibitory activity. The stilbene contents and this activity were moderately to strongly anti-correlated (R = &ndash
0.828, p &le
0.05 for trans-resveratrol). The enzyme kinetic studies revealed a mixed type of inhibition. This study brings promising results for the therapeutic potential of seed and skin Tannat grape extracts as a functional food product with anti-diabetic activity.
Databáze: OpenAIRE
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