RP11‐81H3.2 promotes gastric cancer progression through miR‐339‐HNRNPA1 interaction network
| Autor: | Miao An, Fenrong Chen, Yan Wang, Su-Mei Sha, Dong Liu, Jun Zhang, Shen-hao Wang, Yan Cheng, Haitao Shi, Lei Dong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cancer Research Heterogeneous Nuclear Ribonucleoprotein A1 Apoptosis Metastasis Mice 0302 clinical medicine Cell Movement Tumor Cells Cultured Original Research Cancer Biology Mice Inbred BALB C HNRNPA1 Gene knockdown Chemistry Middle Aged Prognosis lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Gene Expression Regulation Neoplastic Survival Rate Oncology 030220 oncology & carcinogenesis Female RNA Long Noncoding RP11‐81H3.2 Mice Nude miR‐339 lcsh:RC254-282 03 medical and health sciences Stomach Neoplasms In vivo microRNA Biomarkers Tumor medicine Animals Humans Protein Interaction Domains and Motifs Radiology Nuclear Medicine and imaging Cell Proliferation tumor metastasis Oncogene Cell growth gastric cancer Cancer medicine.disease Xenograft Model Antitumor Assays eye diseases MicroRNAs 030104 developmental biology Cell culture Cancer research |
| Zdroj: | Cancer Medicine, Vol 9, Iss 7, Pp 2524-2534 (2020) Cancer Medicine |
| ISSN: | 2045-7634 |
| DOI: | 10.1002/cam4.2867 |
| Popis: | Recent studies have demonstrated that various long non‐coding RNAs (lncRNAs) participate in the gastric cancer (GC) development and metastasis. Some lncRNAs exert their regulatory function by interacting with microRNAs. Here we identified a novel lncRNA RP11‐81H3.2 that was highly expressed in the GC tissue and cell lines. RP11‐81H3.2 knockdown significantly inhibited the proliferation, migration, and invasion of GC cells. Mechanistically, we demonstrated that RP11‐81H3.2 directly interacted with miR‐339 while miR‐339 regulated the HNRNPA1 expression by targeting HRRNPA1 3’‐UTR. RP11‐81H3.2‐miR‐339‐HNRNPA1 interaction network regulated the GC cell proliferation, migration, and invasion. Moreover, our results confirmed that RP11‐81H3.2 knockdown suppressed the tumor growth of GC in a xenograft model in vivo. In summary, the results suggest that RP11‐81H3.2 functions as an oncogene in GC and could be utilized as a promising diagnosis and therapeutic marker for GC treatment. LncRNA RP11‐81H3.2 is highly expressed in gastric cancer tissues and cell lines. Knockdown of lncRNA RP11‐81H3.2 suppresses gastric tumor growth in a xenograft tumor model. RP11‐81H3.2/miR‐339/HNRNPA1 interaction network regulates gastric cancer development and progession. |
| Databáze: | OpenAIRE |
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