Morphological alterations of the reticular thalamic nucleus in Engrailed-2 knockout mice
| Autor: | Cristina Viaggi, Vincenzo Miragliotta, Francesca Vaglini, Elisabetta Giannessi, Andrea Pirone, C Pardini, Carlo Cantile |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty engrailed Histology Autism Spectrum Disorder Thalamus Cell gamma-aminobutyric acid Nerve Tissue Proteins Biology gamma-Aminobutyric acid 03 medical and health sciences Mice 0302 clinical medicine Sex Factors Internal medicine parvalbumin medicine Animals Molecular Biology Ecology Evolution Behavior and Systematics mouse reticular thalamic nucleus Homeodomain Proteins Mice Knockout Neurons Cell Biology Original Articles Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure Parvalbumins Cerebral cortex Thalamic Nuclei Knockout mouse biology.protein GABAergic autism spectrum disorder Female Anatomy Nucleus 030217 neurology & neurosurgery Parvalbumin Developmental Biology medicine.drug |
| Zdroj: | J Anat |
| ISSN: | 1469-7580 |
| Popis: | The reticular thalamic nucleus (Rt) is a sheet of neurons that surrounds the dorsal thalamus laterally, along its dorso‐ventral and rostro‐caudal axes. It consists of inhibitory neurons releasing gamma‐aminobutyric acid (GABA). This nucleus participates in the circuitry between the thalamus and the cerebral cortex, and its impairment is associated with neuro‐psychiatric disorders. In this study, we investigated the Rt anatomy of Engrailed‐2 knockout mice (En2(−/−)), a mouse model of autism spectrum disorder (ASD), using parvalbumin as an immunohistochemical marker. We compared 4‐ and 6‐week‐old wild type (WT) and En2(−/−) mice using various morphometric parameters: cell area, shape factor, circularity and cell density. Significant differences were present in 6‐week‐old male mice with different genetic background (WT vs. En2(−/−)): the Rt neurons of En2 (−/−) mice showed a bigger cell area, shape factor and circularity when compared with WT. Age (4 weeks vs. 6 weeks) influenced the shape factor of WT females, the circularity and cell density of En2 (−/−) males, and the shape factor and circularity of En2 (−/−) females. Gender affected cell density in 4‐week‐old WT mice, shape factor and cellularity of 6‐week‐old WT mice, and cell area, shape factor and cell density of En2 (−/−) at 6 weeks. Intrasubject (left–right) asymmetry of Rt was never observed. These results show for the first time that sex‐ and age‐related changes occur in the Rt GABAergic neurons of the En2 (−/−) ASD mouse model. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text