Mitochondria-Targeted Reaction-Based Fluorescent Probe for Hydrogen Sulfide
| Autor: | Yen Leng Pak, Jun Li, Juyoung Yoon, Gyoungmi Kim, Jin Yong Lee, Kyoung Chul Ko |
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| Rok vydání: | 2016 |
| Předmět: |
Cell Survival
Hydrogen sulfide Nanotechnology 010402 general chemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Humans Moiety Hydrogen Sulfide Fluorescent Dyes Detection limit Microscopy Confocal 010405 organic chemistry Chemistry Combinatorial chemistry Fluorescence Mitochondria 0104 chemical sciences Naphthalimides Piperazine Spectrometry Fluorescence Thiolysis Amine gas treating Selectivity HeLa Cells |
| Zdroj: | ResearcherID |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.6b00956 |
| Popis: | In this study, we developed a turn-on mitochondria-targeting hydrogen sulfide, "probe 1", based on the selective thiolysis of 7-nitro-1,2,3-benzoxadiazole amine moiety attached to the piperazine-based naphthalimide scaffold. Probe 1 exhibited excellent properties with 68-fold fluorescence enhancement, a low detection limit (2.46 μM), a low cytotoxicity, and a good selectivity toward hydrogen sulfide. The success of intracellular imaging indicated that probe 1 could be used in further applications for the investigation of biological functions and pathological roles of H2S in living systems. |
| Databáze: | OpenAIRE |
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