Single-component near-infrared optogenetic systems for gene transcription regulation
| Autor: | Kaberniuk Aa, Daria M. Shcherbakova, Vladislav V. Verkhusha, Mikhail Baloban, Mikhail Monakhov |
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| Přispěvatelé: | Department of Anatomy, Medicum |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
EXPRESSION
Transcription Genetic Expression systems Infrared Rays Science Protein design General Physics and Astronomy Channelrhodopsin Optogenetics General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Mice 0302 clinical medicine Bacterial Proteins Transcription (biology) Protein biosynthesis Transcriptional regulation Animals Humans Psychological repression Synthetic biology Cells Cultured 030304 developmental biology Neurons 0303 health sciences Multidisciplinary Spectroscopy Near-Infrared SPATIOTEMPORAL CONTROL Phytochrome Chemistry LOCALIZATION General Chemistry PHYTOCHROME Cell biology FLUORESCENT PROTEINS Luminescent Proteins Gene Expression Regulation ALL-OPTICAL ELECTROPHYSIOLOGY Female BIOSENSORS 3111 Biomedicine 030217 neurology & neurosurgery Gammaproteobacteria HeLa Cells |
| Zdroj: | Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021) |
| ISSN: | 2041-1723 |
| Popis: | Near-infrared (NIR) optogenetic systems for transcription regulation are in high demand because NIR light exhibits low phototoxicity, low scattering, and allows combining with probes of visible range. However, available NIR optogenetic systems consist of several protein components of large size and multidomain structure. Here, we engineer single-component NIR systems consisting of evolved photosensory core module of Idiomarina sp. bacterial phytochrome, named iLight, which are smaller and packable in adeno-associated virus. We characterize iLight in vitro and in gene transcription repression in bacterial and gene transcription activation in mammalian cells. Bacterial iLight system shows 115-fold repression of protein production. Comparing to multi-component NIR systems, mammalian iLight system exhibits higher activation of 65-fold in cells and faster 6-fold activation in deep tissues of mice. Neurons transduced with viral-encoded iLight system exhibit 50-fold induction of fluorescent reporter. NIR light-induced neuronal expression of green-light-activatable CheRiff channelrhodopsin causes 20-fold increase of photocurrent and demonstrates efficient spectral multiplexing. Current near-IR optogenetic systems to regulate transcription consist of a number of large protein components. Here the authors report a smaller single-component near-IR system, iLight, developed from a bacterial phytochrome that they use to control gene transcription in bacterial and mammalian cells. |
| Databáze: | OpenAIRE |
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