Expression of IL-6 mRNA in normal rat and human pituitaries and in human pituitary adenomas
| Autor: | R. Hooghe, J D'Haens, Brigitte Velkeniers, E L Hooghe-Peters, J Trouillas, Patricia Vergani |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adenoma
Lipopolysaccharides medicine.medical_specialty Pituitary gland Time Factors Histology Palatine Tonsil In situ hybridization Biology Paracrine signalling Adrenocorticotropic Hormone Internal medicine Gene expression medicine Animals Humans Pituitary Neoplasms RNA Messenger Rats Wistar Autocrine signalling Cells Cultured In Situ Hybridization Interleukin-6 Lymphokine Receptors Interleukin medicine.disease Immunohistochemistry Receptors Interleukin-6 Rats Endocrinology medicine.anatomical_structure Growth Hormone Pituitary Gland Female Anatomy Spleen Endocrine gland |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 42:67-76 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/42.1.8263325 |
| Popis: | Cells expressing IL-6 mRNA were detected by in situ hybridization in normal pituitaries. In normal untreated rat pituitary the expression was very low. Within hours after IP administration of liposaccharide, IL-6 mRNA accumulated in the anterior lobe of the pituitary. Production of IL-6 was monitored after dissociation and culture of pituitary cells. High levels (8000 pg/ml) were recovered after 72 hr in culture. In normal human pituitaries, less than 1% of cells expressed IL-6 mRNA or IL-6 receptor mRNA (IL-6-R mRNA). In gonadotropinomas, prolactinomas, and non-functioning adenomas, only rare, scattered positive cells were found for either IL-6 or IL-6-R. In contrast, both genes were highly expressed in ACTH- and GH-secreting tumors at the junction of adenoma and infiltrating fibrous tissue and around blood vessels. The combined expression of IL-6 and IL-6-R suggests that IL-6 acts in an autocrine or in a paracrine way in ACTH and GH adenomas. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|