Evaluation of the Cytotoxic and Autophagic Effects of Atorvastatin on MCF-7 Breast Cancer Cells
| Autor: | Naciye Dilara Zeybek, Sevda Muftuoglu, Tugba Alarcon Martínez |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
autophagy Programmed cell death lcsh:Medicine Breast Neoplasms Apoptosis Apoptosis atorvastatin autophagy breast cancer cytotoxicity MCF-7 cells 03 medical and health sciences breast cancer 0302 clinical medicine Breast cancer medicine Humans Cytotoxic T cell MCF-7 cells Viability assay Cell Proliferation Chemistry Cell growth lcsh:R Autophagy atorvastatin General Medicine medicine.disease Gene Expression Regulation Neoplastic 030104 developmental biology MCF-7 030220 oncology & carcinogenesis Cancer research cytotoxicity Original Article Female |
| Zdroj: | Balkan Medical Journal, Vol 35, Iss 3, Pp 256-262 (2018) Balkan Medical Journal Volume: 35, Issue: 3 256-262 |
| ISSN: | 2146-3131 2146-3123 |
| DOI: | 10.4274/balkanmedj.2017.0604 |
| Popis: | Background: Recently, cytotoxic effects of statins on breast cancer cells have been reported. However, the mechanism of antiproliferative effects is currently unknown. Autophagy is non-apoptotic programmed cell death, which is characterized by degradation of cytoplasmic components and as having a role in cancer pathogenesis. Aims: To investigate the anti-proliferative effects of atorvastatin on MCF-7 human breast adenocarcinoma cells with respect to both autophagy and apoptosis. Study Design: Cell culture study. Methods: Cell viability was analyzed using WST-1 cell proliferation assay. Apoptosis was determined by the TUNEL method, whereas autophagy was assessed by Beclin-1 and LC3B immunofluorescence staining. Ultrastructural analysis of cells was performed by electron microscopy. Results: Atorvastatin reduced MCF-7 cell proliferation in a dose- and time-dependent manner inducing TUNEL-, Beclin-1-, and LC3Bpositive cells. Moreover, ultrastructural analysis showed apoptotic, autophagic, and necrotic morphological changes in treatment groups. A statistically significant increase in the apoptotic index was detected with higher concentrations of atorvastatin at 24 h and 48 h (p |
| Databáze: | OpenAIRE |
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