Hydrolysis of proteins by successive incubation with proteinase and aminopeptidases mixture
| Autor: | Masaru Iizuka, Takehiko Yamamoto, Toshihiro Nakanishi, Yoshikazu Matsumura |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Cystine Bacillus subtilis biology.organism_classification Streptomyces General Biochemistry Genetics and Molecular Biology Amino acid chemistry.chemical_compound Hydrolysis Enzyme Biochemistry chemistry General Agricultural and Biological Sciences Glycoprotein Cysteine |
| Zdroj: | Agricultural and Biological Chemistry. 39:379-386 |
| ISSN: | 1881-1280 0002-1369 |
| Popis: | 1. A trial test was attempted of complete hydrolysis of peptides and proteins into amino acids by enzymes. “Neutral proteinase” of Bacillus subtilis or “Alkalophilic proteinase” of a Streptomyces sp. was used for preliminary digestion of substrate, and a mixture of three aminopeptidases of Bacillus subtilis was employed for subsequent hydrolysis of proteinase digest.2. The oxidized insulin B chain was hydrolyzed completely by the method. Several proteins including enzymes which contained no or less cystine and cysteine were also hydrolyzed almost completely.3. On the other hand, certain glycoproteins were hydrolyzed to leave a few glycopeptides in which all glycomoieties of the proteins were retained. The implications of the results are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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