Cyclosporine A – Induced gingival overgrowth and proliferating cell nuclear antigen expression in experimental periodontitis
| Autor: | Yasmin Rodarte Carvalho, Lucilene Hernandes Ricardo, Debora Pallos, Felipe da Silva Peralta, Renata Falchete do Prado |
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| Přispěvatelé: | Periodontics Department, Universidade Estadual Paulista (UNESP), UNISA - Santo Amaro University |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Pathology
medicine.medical_specialty Proliferative index medicine.medical_treatment Inflammation Article 03 medical and health sciences Cyclosporine A 0302 clinical medicine Epithelial hyperplasia Statistical significance Gingival overgrowth medicine PCNA Ligature General Dentistry Periodontitis biology business.industry Significant difference Immunohistochemical analysis 030206 dentistry medicine.disease Proliferating cell nuclear antigen Otorhinolaryngology 030220 oncology & carcinogenesis biology.protein Immunohistochemistry medicine.symptom business |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-29T08:27:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The most important microscopic characteristic of Cyclosporine A-induced gingival overgrowth is fibroepithelial hyperplasia. Objective: The objective was to investigate the influence of previous exposure to Cyclosporine A over gingival epithelium in experimental periodontitis in rats. Methods: Twenty Wistar rats with 12 weeks-old were divided into four groups with 5 animals each: Control Group (CG); Cyclosporine Group (CsAG); Ligature group (LG) and Cyclosporine and Ligature Group (CsALG). Daily doses of CsA (10 mg/kg) were applied to CsAG and CsALG during 60 days since the beginning of the experiment and, a ligature was placed in LG and CsALG 30 days after the beginning of the experiment. After 60 days, animals were euthanized and gingival tissue was processed to histomorphometric analysis of epithelial thickness (mm2), immunohistochemical expression of PCNA (%) and inflammatory response. Data were analyzed by Kruskal-Wallis and Mann Whitney at 0.05 significance level. Results: Considering epithelial thickness, CG was thinner than all groups, CsALG was the largest and CsAG and LG were similar between each other. Regarding the PCNA expression CG (16.46 ± 9.26) was similar to CsAG (34.47 ± 19.75) and, LG (59.02 ± 10.33) was similar to CsALG (40.59 ± 18.25). Significant difference (p < 0.05) occurred only in inflammation presence comparing CG/LG and CsAG/CsALG. A weak positive correlation between the number of PCNA+ and inflammatory cells (p = 0.001; r = 0.611) was observed. Conclusion: Based on these results it was concluded that the enlargement of gingival epithelium observed in experimental periodontitis can be increased by previous exposition to CsA and inflammatory conditions enhanced proliferative activity of the keratinocytes. Periodontics Department Department of Dentistry of Universiy of Taubaté Departament of Oral Biopathology of Institute of Science and Tecnology São Paulo State University, São José Dos Campos UNISA - Santo Amaro University Departament of Oral Biopathology of Institute of Science and Tecnology São Paulo State University, São José Dos Campos FAPESP: 08/54784-4 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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