Establishment and characterization of immortalized bovine male germline stem cell line
| Autor: | Jinlian Hua, Qijing Lei, Shulin Chen, Ju-hong Ma, Guang-peng Li, Qin Pan, Zhe Zhou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
proliferation Agriculture (General) Plant Science Germ layer testis Biology immortalization Biochemistry Germline S1-972 03 medical and health sciences male germline stem cells Food Animals Antigen cell transplantation Ecology bovine In vitro Cell biology Transplantation 030104 developmental biology Cell culture Immunology Animal Science and Zoology Stem cell Agronomy and Crop Science Spermatogenesis Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 16, Iss 11, Pp 2547-2557 (2017) |
| ISSN: | 2095-3119 |
| DOI: | 10.1016/s2095-3119(16)61625-8 |
| Popis: | Male germline stem cells (mGSCs) are unique adult germ cells with self-renewal potential and spermatogenesis function in the testis. However, further studies are needed to establish a long-term cultural system of mGSCs in vitro, especially for large animals such as bovine mGSCs. In this study, we first established a stable immortalized bovine male germline stem cell line by transducing Simian virus 40 (SV40) large T antigen. The proliferation of these cells was improved significantly. These cells could express spermatogonial stem cell (SSC)-specific markers, such as PLZF, PGP9.5, VASA, LIN28A, and CD49F, both in the mRNA and protein levels. Additionally, these cells could be differentiated into three germ layer cells to enter meiosis, form colonies, and proliferate in the seminiferous tubules of busulfan-induced infertile mice. The immortalized bovine mGSCs maintain the criteria of mGSCs. |
| Databáze: | OpenAIRE |
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