Nonlinear relation between concentration and fluorescence emission of protoporphyrin IX in calibrated phantoms
| Autor: | Laurent Mahieu-Williame, Bruno Montcel, David Rousseau, Mathieu Hébert, Laure Alston |
|---|---|
| Přispěvatelé: | RMN et optique : De la mesure au biomarqueur, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Images et Modèles, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Plateforme d'Imagerie Multimodale LyonTech (PILoT) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030103 biophysics Materials science Biomedical Engineering Analytical chemistry Protoporphyrins 01 natural sciences Spectral line Fluorescence spectroscopy Imaging phantom 010309 optics Biomaterials 03 medical and health sciences chemistry.chemical_compound 0103 physical sciences Emission spectrum ComputingMilieux_MISCELLANEOUS Protoporphyrin IX Phantoms Imaging Aminolevulinic Acid Fluorescence Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Wavelength Spectrometry Fluorescence chemistry Nonlinear Dynamics Calibration [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic Luminescence |
| Zdroj: | Journal of Biomedical Optics Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2018, 23 (09), ⟨10.1117/1.JBO.23.9.097002⟩ |
| ISSN: | 1560-2281 1083-3668 |
| Popis: | 5-ALA-induced protoporphyrin IX (PpIX) has shown its relevance in medical assisting techniques, notably in the detection of glioma (brain tumors). Validation of instruments on phantoms is mandatory and a standardization procedure has recently been proposed. This procedure yields phantoms recipes to realize a linear relationship between PpIX concentration and fluorescence emission intensity. The present study puts forward phantoms where this linear relationship cannot be used. We propose a model that considers two states of PpIX, corresponding to two different aggregates of PpIX, with fluorescence spectra peaking at 634 and 620 nm, respectively. We characterize the influence of these two states on PpIX fluorescence emission spectra in phantoms with steady concentration of PpIX and various microenvironment parameters (surfactant, Intralipid or bovine blood concentration, and pH). We show that, with fixed PpIX concentration, a modification of the microenvironment induces a variation of the emitted spectrum, notably a shift in its central wavelength. We show that this modification reveals a variation of proportions of the two states. This establishes phantom microenvironment regimes where the usual single state model is biased while a linear combination of the two spectra enables accurate recovering of any measured spectra. |
| Databáze: | OpenAIRE |
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