'Neighbourhood watch' model: embryonic epiblast cells assess positional information in relation to their neighbours
| Autor: | Nidia M.M. Oliveira, Karen M. Page, Wolpert L, Cato Hastings, Ruben Perez-Carrasco, Hyung Chul Lee, Claudio D. Stern |
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| Rok vydání: | 2021 |
| Předmět: |
animal structures
Primitive streak formation Polarity Gastrulation Embryo Chick Embryo Gastrula Biology 06 Biological Sciences Chick embryos Embryonic stem cell Cell communication Epiblast embryonic structures Animals Molecular Biology Neuroscience Morphogen Primitive streak SMAD 11 Medical and Health Sciences Germ Layers Developmental Biology |
| Zdroj: | Development (Cambridge, England). 149(10) |
| ISSN: | 1477-9129 |
| Popis: | In many developing and regenerating systems, tissue pattern is established through gradients of informative morphogens, but we know little about how cells interpret these. Using experimental manipulation of early chick embryos including misexpression of an inducer (VG1 or ACTIVIN) and an inhibitor (BMP4), we test two alternative models for their ability to explain how the site of primitive streak formation is positioned relative to the rest of the embryo. In one model, cells read morphogen concentrations cell-autonomously. In the other, cells sense changes in morphogen status relative to their neighbourhood. We find that only the latter model can account for the experimental results, including some counter-intuitive predictions. This mechanism (which we name “neighbourhood watch” model) illuminates the classic “French Flag Problem” and how positional information is interpreted by a sheet of cells in a large developing system.Summary statementIn a large developing system, the chick embryo before gastrulation, cells interpret gradients of positional signals relative to their neighbours to position the primitive streak, establishing bilateral symmetry. |
| Databáze: | OpenAIRE |
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