Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development
| Autor: | Thierry Leroy, David Pot, Luiz Filipe Protasio Pereira, Douglas Silva Domingues, Maria Brígida dos Santos Scholz, Leonardo M. Sakuray, Suzana Tiemi Ivamoto, Cíntia Sorane Good Kitzberger, Luiz Gonzaga Esteves Vieira, Lucia Pires Ferreira |
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| Přispěvatelé: | Laboratório de Biotecnologia Vegetal, Universidade Estadual de Londrina (UEL), Laboratório de Ecofisiologia Vegetal, UMR AGAP, Universidade do Oeste Paulista (UNOESTE), Universidade Estadual Paulista (Unesp), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transcription Genetic Physiology Biosynthèse Cafestol Kahweol Fleur Coffea Plant Science Plant Roots Coffee 01 natural sciences Tissu végétal F30 - Génétique et amélioration des plantes chemistry.chemical_compound Cytochrome P-450 Enzyme System Gene Expression Regulation Plant Gene expression Plant defense against herbivory Diterpénoïde Expression des gènes Chromatography High Pressure Liquid Plant Proteins biology food and beverages Feuille Coffea arabica Composition chimique PCR Diterpenes medicine.drug Développement biologique F60 - Physiologie et biochimie végétale Flowers Genes Plant 03 medical and health sciences Botany Genetics medicine Q04 - Composition des produits alimentaires Genetic Association Studies Aroma RT-qPCR Cytochrome P450 biology.organism_classification Plant Leaves 030104 developmental biology chemistry Fruit biology.protein Diterpene U30 - Méthodes de recherche HPLC 010606 plant biology & botany Racine |
| Zdroj: | Plant Physiology and Biochemistry Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T16:45:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-02-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30–240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways. Instituto Agronômico do Paraná (IAPAR) Laboratório de Biotecnologia Vegetal, CP 481 Universidade Estadual de Londrina (UEL), CP 6001 Instituto Agronômico do Paraná (IAPAR) Laboratório de Ecofisiologia Vegetal, CP 481 Centre de Coopération Internationale en Recherche Agronomique Pour le Développement (CIRAD) UMR AGAP Universidade do Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572 Universidade Estadual Paulista (UNESP) Instituto de Biociências de Rio Claro, Avenida 24-A, 1515 Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA Café) Universidade Estadual Paulista (UNESP) Instituto de Biociências de Rio Claro, Avenida 24-A, 1515 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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