Quantification of intracellular HNO delivery with capillary zone electrophoresis
| Autor: | Katrina M. Miranda, Thilini N. Amarakoon, Neng Ke, Craig A. Aspinwall |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
chemistry.chemical_classification
Cancer Research Reactive oxygen species Physiology Chemistry Clinical Biochemistry Electrophoresis Capillary Nitroxyl Glutathione Biochemistry Redox Small molecule Article chemistry.chemical_compound Capillary electrophoresis Cell Line Tumor Biophysics Humans Nitrogen Oxides Signal transduction Function (biology) Intracellular |
| Zdroj: | Nitric Oxide |
| ISSN: | 1089-8603 |
| DOI: | 10.1016/j.niox.2021.10.005 |
| Popis: | Redox signaling, wherein reactive and diffusible small molecules are channeled into specific messenger functions, is a critical component of signal transduction. A central principle of redox signaling is that the redox modulators are produced in a highly controlled fashion to specifically modify biotargets. Thiols serve as primary mediators of redox signaling as a function of the rich variety of adducts, which allows initiation of distinct cellular effects. Coupling the inherent reactivity of thiols with highly sensitive and selective chemical analysis protocols can facilitate identification of redox signaling agents, both in solution and in cultured cells. Here, we describe use of capillary zone electrophoresis to both identify and quantify sulfinamides, which are specific markers of the reaction of thiols with nitroxyl (HNO), a putative biologically relevant reactive nitrogen species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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