Plasma and memory B cell responses targeting O-specific polysaccharide (OSP) are associated with protection against Vibrio cholerae O1 infection among household contacts of cholera patients in Bangladesh
| Autor: | Taher Uddin, Richelle C. Charles, Firdausi Qadri, Anjali Mandlik, Tahirah Yasmin, Edward T. Ryan, Jason B. Harris, Sultana Rownok Jahan, Peng Xu, Aklima Akter, Amena Aktar, Pavol Kováč, Sadia Afrin, Fahima Chowdhury, Meagan Kelly, Md. Israk Nur Sami, Stephen B. Calderwood, Taufiqur Rahman Bhuiyan, M. Arifur Rahman, Ashraful Islam Khan, Pinki Dash, Regina C. LaRocque |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Bacterial Diseases Lipopolysaccharide Physiology Antibody Response medicine.disease_cause Pathology and Laboratory Medicine Biochemistry chemistry.chemical_compound Plasma 0302 clinical medicine Medical microbiology Cholera Immune Physiology Cellular types Medicine and Health Sciences Enzyme-Linked Immunoassays Memory B cell Child Immune Response B-Lymphocytes Bangladesh Family Characteristics Immune System Proteins biology lcsh:Public aspects of medicine Immune cells Vibrio cholerae O1 O Antigens Middle Aged Antibodies Bacterial 3. Good health Bacterial Pathogens Body Fluids Infectious Diseases Blood Vibrio cholerae Medical Microbiology Child Preschool White blood cells Female Antibody Pathogens Anatomy Research Article Neglected Tropical Diseases Adult medicine.medical_specialty Cell biology Blood cells lcsh:Arctic medicine. Tropical medicine Adolescent lcsh:RC955-962 030231 tropical medicine Immunology Antibody-producing cells Research and Analysis Methods Microbiology Blood Plasma Antibodies 03 medical and health sciences Young Adult Immune system Vibrio Cholerae medicine Humans Immunoassays Microbial Pathogens Vibrio B cells Biology and life sciences Bacteria business.industry Public Health Environmental and Occupational Health Organisms Correction Proteins lcsh:RA1-1270 biology.organism_classification medicine.disease Tropical Diseases Memory B cells Immunoglobulin A 030104 developmental biology chemistry Immunoglobulin M Animal cells Immunoglobulin G biology.protein Immunologic Techniques business Immunologic Memory |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Vol 12, Iss 4, p e0006399 (2018) |
| ISSN: | 1935-2735 1935-2727 |
| Popis: | Background The mediators of protection against cholera, a severe dehydrating illness of humans caused by Vibrio cholerae, are unknown. We have previously shown that plasma IgA as well as memory B IgG cells targeting lipopolysaccharide (LPS) of Vibrio cholerae O1 correlate with protection against V. cholerae O1 infection among household contacts of cholera patients. Protection against cholera is serogroup specific, and serogroup specificity is defined by the O-specific polysaccharide (OSP) component of LPS. Therefore, we prospectively followed household contacts of cholera patients to determine whether OSP-specific immune responses present at the time of enrollment are associated with protection against V. cholerae infection. Methodology In this study, we enrolled two hundred forty two household contacts of one hundred fifty index patients who were infected with Vibrio cholerae. We determined OSP-specific memory B cells and plasma IgA, IgG and IgM antibody responses on study entry (day 2). Principle findings The presence of OSP-specific plasma IgA, IgM, and IgG antibody responses on study entry were associated with a decrease in the risk of infection in household contacts (IgA, p = 0.015; IgM, p = 0.01, and IgG, p = 0.024). In addition, the presence of OSP-specific IgG memory B cell responses in peripheral blood on study entry was also associated with a decreased risk of infection (44% reduction; 95% CI: 31.1 to 99.8) in contacts. No protection was associated with cholera toxin B subunit (CtxB)-specific memory B cell responses. Conclusion These results suggest that immune responses that target OSP, both in plasma and memory responses, may be important in mediating protection against infection with V. cholerae O1. Author summary Vibrio cholerae is a non-invasive pathogen which causes watery diarrheal diseases both in adults and children. Natural infection with Vibrio cholerae provides protection against subsequent diseases and protection against cholera is serogroup specific. Serogroup specificity is defined by O-specific polysaccharide (OSP) of V. cholerae. In this study, we have found that uninfected household contacts had higher baseline OSP-specific plasma IgA, IgG and IgM antibody responses than infected contacts. These observations demonstrate those plasma antibodies responses against OSP are associated with a decrease of the risk of infection of household contacts of cholera patients. We also found that OSP-specific IgG memory B cells are associated with a decrease in the risk of infection in contacts of cholera patients. This result further supports the hypothesis that immune responses targeting V. cholerae OSP is a prime mediator of protection against cholera, and suggests that future work should focus on more detailed analysis of mucosal immune responses targeting OSP, as well as evaluation of potential mechanisms of how antibodies targeting V. cholerae OSP might mediate protection against cholera. |
| Databáze: | OpenAIRE |
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