Identification and Expression Analysis of GRAS Transcription Factors to Elucidate Candidate Genes Related to Stolons, Fruit Ripening and Abiotic Stresses in Woodland Strawberry (

Autor: Jing Liu, Xiaoqing Lu, Han Wu, Huihui Li, H. S. Chen, Longzheng Chen
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Rosaceae
Arabidopsis
genome-wide identification
01 natural sciences
lcsh:Chemistry
Gene Expression Regulation
Plant
non-climacteric fruit ripening
lcsh:QH301-705.5
Spectroscopy
Phylogeny
Abiotic component
biology
Stolon
food and beverages
Ripening
General Medicine
Fragaria
Computer Science Applications
stolons
Gibberellin
gibberellins
Plant Development
Catalysis
Article
Inorganic Chemistry
Evolution
Molecular
03 medical and health sciences
Woodland Strawberry
Stress
Physiological
Botany
expression analysis
GRAS transcription factors
Physical and Theoretical Chemistry
Molecular Biology
Gene
Cold-Shock Response
Organic Chemistry
fungi
Oryza
biology.organism_classification
abiotic stresses
woodland strawberry
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Fruit
Heat-Shock Response
010606 plant biology & botany
Transcription Factors
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 18
International Journal of Molecular Sciences, Vol 20, Iss 18, p 4593 (2019)
ISSN: 1422-0067
Popis: The cultivated strawberry (Fragaria ×
ananassa), an allo-octoploid with non-climacteric fleshy fruits, is a popular Rosaceae horticultural crop worldwide that is mainly propagated via stolons during cultivation. Woodland strawberry (Fragaria vesca), one of the four diploid progenitor species of cultivated strawberry, is widely used as a model plant in the study of Rosaceae fruit trees, non-climacteric fruits and stolons. One GRAS transcription factor has been shown to regulate stolon formation
the other GRAS proteins in woodland strawberry remain unknown. In this study, we identified 54 FveGRAS proteins in woodland strawberry, and divided them into 14 subfamilies. Conserved motif analysis revealed that the motif composition of FveGRAS proteins was conserved within each subfamily, but diverged widely among subfamilies. We found 56 orthologous pairs of GRAS proteins between woodland strawberry and Arabidopsis thaliana, 47 orthologous pairs between woodland strawberry and rice and 92 paralogous pairs within woodland strawberry. The expression patterns of FveGRAS genes in various organs and tissues, and changes therein under cold, heat and GA3 treatments, were characterized using transcriptomic analysis. The results showed that 34 FveGRAS genes were expressed with different degrees in at least four organs, including stolons
only a few genes displayed organ-specific expression. The expression levels of 16 genes decreased, while that of four genes increased during fruit ripening
FveGRAS54 showed the largest increase in expression. Under cold, heat and GA3 treatments, around half of the FveGRAS genes displayed increased or decreased expression to some extent, suggesting differing functions of these FveGRAS genes in the responses to cold, heat and GAs. This study provides insight into the potential functions of FveGRAS genes in woodland strawberry. A few FveGRAS genes were identified as candidate genes for further study, in terms of their functions in stolon formation, fruit ripening and abiotic stresses.
Databáze: OpenAIRE
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