Bottom-Up Design Approach for OBOC Peptide Libraries
| Autor: | Daniela Kalafatovic, Ernest Giralt, Dina Rešetar Maslov, Goran Mausa |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Solid-phase synthesis
Computer science Pharmaceutical Science Química combinatòria Peptide Combinatorial chemistry 010402 general chemistry 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 lcsh:Organic chemistry Peptide Library Tandem Mass Spectrometry Drug Discovery Combinatorial Chemistry Techniques Amino Acid Sequence Physical and Theoretical Chemistry Amino Acids Chromatography High Pressure Liquid Solid-Phase Synthesis Techniques chemistry.chemical_classification Sequence 010405 organic chemistry Organic Chemistry Top-down and bottom-up design Síntesi de pèptids Chemical space Microspheres UPLC-MS analysis combinatorial Síntesi en fase sólida 0104 chemical sciences OBOC peptide libraries bottom-up design Peptide synthesis chemistry Chemistry (miscellaneous) Molecular Medicine Biochemical engineering Peptides Algorithms |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Molecules Volume 25 Issue 15 Molecules, Vol 25, Iss 3316, p 3316 (2020) |
| Popis: | One-bead-one-compound peptide libraries, developed following the top-down experimental approach, have attracted great interest in the identification of potential ligands or active peptides. By exploiting a reverse experimental design approach based on the bottom-up strategy, we aimed to develop simplified, maximally diverse peptide libraries that resulted in the successful characterization of mixture components. We show that libraries of 32 and 48 components can be successfully detected in a single run using chromatography coupled to mass spectrometry (UPLC-MS). The proposed libraries were further theoretically evaluated in terms of their composition and physico-chemical properties. By combining the knowledge obtained on single libraries we can cover larger sequence spaces and provide a controlled exploration of the peptide chemical space both theoretically and experimentally. Designing libraries by using the bottom-up approach opens up the possibility of rationally fine-tuning the library complexity based on the available analytical methods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|