Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species
| Autor: | Stuart C. Clarke, Jeanne-Marie Devaster, Nathalie Devos, Tom Wilkinson, Karen L Osman, Christopher H. Woelk, Marie-Cécile Mortier, Thierry G. Pascal, David W. Cleary, Johanna M.C. Jefferies |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Haemophilus Infections In silico 030106 microbiology lcsh:Medicine Polymerase Chain Reaction Genome Article law.invention Pulmonary Disease Chronic Obstructive 03 medical and health sciences Phylogenetics law Molecular genetics Haemophilus medicine otorhinolaryngologic diseases Humans Allele lcsh:Science Phylogeny Polymerase chain reaction Aged Aged 80 and over Genetics Multidisciplinary biology lcsh:R Phosphotransferases Middle Aged biochemical phenomena metabolism and nutrition Haemolysis biology.organism_classification bacterial infections and mycoses Haemophilus influenzae 3. Good health bacteria lcsh:Q Female |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| Popis: | H. haemolyticus is often misidentified as NTHi due to their close phylogenetic relationship. Differentiating between the two is important for correct identification and appropriate treatment of infective organism and to ensure any role of H. haemolyticus in disease is not being overlooked. Speciation however is not completely reliable by culture and PCR methods due to the loss of haemolysis by H. haemolyticus and the heterogeneity of NTHi. Haemophilus isolates from COPD as part of the AERIS study (ClinicalTrials - NCT01360398) were speciated by analysing sequence data for the presence of molecular markers. Further investigation into the genomic relationship was carried out using average nucleotide identity and phylogeny of allelic and genome alignments. Only 6.3% were identified as H. haemolyticus. Multiple in silico methods were able to distinguish H. haemolyticus from NTHi. However, no single gene target was found to be 100% accurate. A group of omp2 negative NTHi were observed to be phylogenetically divergent from H. haemolyticus and remaining NTHi. The presence of an atypical group from a geographically and disease limited set of isolates supports the theory that the heterogeneity of NTHi may provide a genetic continuum between NTHi and H. haemolyticus. |
| Databáze: | OpenAIRE |
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