Overexpression of the actin gene is associated with the morphogenesis of Candida albicans
| Autor: | Asis Datta, Vijay Paranjape |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Time Factors
Calmodulin Biophysics Morphogenesis Germ tube Saccharomyces cerevisiae Biochemistry Acetylglucosamine Gene Expression Regulation Fungal Candida albicans Gene expression RNA Messenger Northern blot Molecular Biology Actin biology Temperature Cell Biology Blotting Northern biology.organism_classification Molecular biology Actins Trifluoperazine In vitro Glucose Protein Biosynthesis biology.protein |
| Zdroj: | Biochemical and Biophysical Research Communications. 179:423-427 |
| ISSN: | 0006-291X |
| Popis: | A progressive increase in the synthesis of actin mRNA was observed by Northern blot analysis, when cells were induced to form germ tubes at 37 degrees C by N-acetyl-D-glucosamine. Presence of trifluoperazine, a calmodulin inhibitor, or incubation of cells at 25 degrees C, or by replacing N-acetyl-D-glucosamine with glucose which inhibited germ tube formation lowered this synthesis. Furthermore, in vitro translation of total RNA revealed an increase in the synthesis of actin (45 kDa) during germ tube formation. These results suggest for the first time that the expression of actin gene is regulated during morphogenesis of C. albicans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|