Capillary electrophoresis as a simple and sensitive method to study polysaccharides of Sinorhizobium sp. NGR234
| Autor: | François Couderc, Véréna Poinsot, Christel Vérollet, Nicolas Fraysse |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Clinical Biochemistry Monosaccharides Polysaccharides Bacterial Electrophoresis Capillary Oligosaccharides Sinorhizobium Polysaccharide Mass spectrometry biology.organism_classification Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry Cell wall Hydrolysis Capillary electrophoresis Monosaccharide Laser-induced fluorescence Chromatography High Pressure Liquid |
| Zdroj: | Electrophoresis. 24(19-20) |
| ISSN: | 0173-0835 |
| Popis: | We report the minimum amounts of polysaccharides (6.5 ng) that can be studied using capillary electrophoresis-laser-induced fluorescence (CE-LIF) and describe the separation of the various monosaccharides susceptible to occur in Sinorhizobium cell wall. On-gel hydrolysis of the polysaccharides is described. It allowed the easy and rapid determination of the monosaccharide composition of the excised lipopolysaccharides. The composition obtained fits with gas chromatography-mass spectrometry (GC-MS) results. This study underlines the simplicity of using CE-LIF, which remains an entirely "water medium" method, unlike GC-MS which is, in addition, less sensitive (at least 100-fold) than CE-LIF. |
| Databáze: | OpenAIRE |
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