Glycosyl hydrolases from Bifidobacterium adolescentis DSM20083. An overview
| Autor: | Jean-Paul Vincken, Sandra W.A. Hinz, Alphons G. J. Voragen, Lambertus A.M. van den Broek, Gerrit Beldman, Chantal H. L. Doeswijk-Voragen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_treatment
genome sequence Biology Stachyose chemistry.chemical_compound oligosaccharides Levensmiddelenchemie medicine Glycoside hydrolase Glycosyl Raffinose sucrose phosphorylase Bifidobacterium VLAG chemistry.chemical_classification catalytic nucleophile Food Chemistry Prebiotic Sucrose phosphorylase Oligosaccharide biology.organism_classification dsm 20083 chemistry Biochemistry probiotics alpha-galactosidase beta-galactosidases active-center prebiotics Food Science |
| Zdroj: | Lait 85 (2005) 1-2 Lait, 85(1-2), 125-133 |
| ISSN: | 0023-7302 |
| Popis: | It is claimed that bifidobacteria have several health-promoting effects. To increase the amount of bifidobacteria in the colon the concept of probiotics and/or prebiotics can be applied. Bifi- dobacterium adolescentis is one of the main species of bifidobacteria in the gastro-intestinal tract of human adults. B. adolescentis is able to degrade a wide range of oligosaccharides and a number of glycosyl hydrolases have been characterized in detail. The hydrolytic activity of the glycosyl hydrolases from B. adolescentis toward prebiotics like arabinoxylan-oligosaccharides, isomalto-oli- gosaccharides, arabinogalactan, and sucrose-based oligosaccharides (raffinose, stachyose, and fructo-oligosaccharides) is reviewed. Alternatively, some of these glycosyl hydrolases are able to catalyze transglycosylation, which allows them to elongate oligosaccharides and to prepare poten- tially prebiotic oligosaccharides. Such oligosaccharides might be used to influence the microbial composition in the more distal parts of the colon. In nature, not all enzyme-substrate encounters are transglycosylating. So, the hydrolytic activity of the enzyme makes the oligosaccharide elongation less efficient than desired. Site-directed mutagenesis was applied to improve the transglycosylation reaction of the α-galactosidase from B. adolescentis. Bifidobacterium / prebiotic / glycosyl hydrolase / transglycosylation / site-directed mutagenesis Resume - Les glycosyl hydrolases de Bifidobacterium adolescentis DSM20083. Il est affirme que les bifidobacteries ont des proprietes benefiques sur la sante. Pour augmenter la teneur en bifido- bacteries au niveau du colon, le concept de probiotiques et/ou prebiotiques peut etre applique. Bifidobacterium adolescentis est l'une des principales especes de bifidobacteries dans le tractus gas- tro-intestinal des adultes. Certaines des glycosyl hydrolases de B. adolescentis ont ete caracterisees en detail et elles semblent degrader un large eventail d'oligosaccharides. L'activite d'hydrolyse des glycosyl hydrolases de B. adolescentis envers des prebiotiques tels que les arabino-xylanes, isomalto- oligosaccharides, arabino-galactanes et les oligosaccharides contenant du saccharose (raffinose, sta- chyose et fructo-oligosaccharides) est passee en revue. De plus, certaines de ces glycosyl hydrolases peuvent etre utilisees pour preparer des oligosaccharides prebiotiques potentiels par transglycosy- lation, ce qui permet d'allonger les oligosaccharides. De tels oligosaccharides peuvent etre utilises pour influencer la composition microbienne au niveau des parties plus distales du colon. Les pro- duits de rencontre enzymes-substrates ne sont pas tous transglycosyles naturellement et l'activite d'hydrolyse de l'enzyme entraine une elongation de l'oligosaccharide moindre que celle desiree. La mutagenese dirigee a ete appliquee pour ameliorer la reaction de transglycosylation de l'α-galactosi- dase de B. adolescentis. Bifidobacterium / prebiotique / glycosyl hydrolases / transglycosylation / mutagenese dirigee |
| Databáze: | OpenAIRE |
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