Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands
| Autor: | L. A. Torpet, Jesper Reibel, Anne Marie Lynge Pedersen, Kim Brøsen, Christian von Buchwald, Camilla Kragelund, Birgitte Nauntofte, Marianne Hamilton Therkildsen, Claus Hansen |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Saliva medicine.medical_specialty Alcohol Drinking Submandibular Gland Salivary Glands Minor Gene Expression Regulation Enzymologic Salivary Glands Xenobiotics Young Adult chemistry.chemical_compound Serous Membrane stomatognathic system Cytochrome P-450 CYP1A2 Internal medicine medicine Cytochrome P-450 CYP3A Humans Parotid Gland Salivary Ducts Salivary Proteins and Peptides Oral mucosa General Dentistry In Situ Hybridization Aged Aged 80 and over Mucous Membrane biology Smoking CYP1A2 Cytochrome P450 Middle Aged Immunohistochemistry Submandibular gland Parotid gland stomatognathic diseases medicine.anatomical_structure Endocrinology Otorhinolaryngology chemistry biology.protein Female Xenobiotic |
| Zdroj: | University of Copenhagen Kragelund, C, Hansen, C, Torpet, L A, Nauntofte, B, Brøsen, K, Pedersen, A M L, Buchwald, C, Therkildsen, M H & Reibel, J 2008, ' Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands ', Oral Diseases, vol. 14, no. 6, pp. 533-40 . https://doi.org/10.1111/j.1601-0825.2007.01415.x |
| ISSN: | 1601-0825 1354-523X |
| DOI: | 10.1111/j.1601-0825.2007.01415.x |
| Popis: | Udgivelsesdato: 2008-Sep OBJECTIVE: The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands. METHODS: Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA. RESULTS: CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent. CONCLUSION: The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva. |
| Databáze: | OpenAIRE |
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