Immunoglobulin framework-derived peptides function as cytotoxic T-cell epitopes commonly expressed in B-cell malignancies
| Autor: | Marco Ladetto, Mathias Witzens, Robert H. Vonderheide, Joachim L. Schultze, Andreas Trojan, John G. Gribben, John W. Donovan |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Immunoglobulin Variable Region
Epitopes T-Lymphocyte Immunoglobulins Lymphoma Mantle-Cell General Biochemistry Genetics and Molecular Biology Epitope Immunity HLA-A2 Antigen medicine Humans Cytotoxic T cell Lymphoma Follicular Cells Cultured B cell Multiple myeloma B-Lymphocytes biology Lymphoma Non-Hodgkin Histocompatibility Antigens Class I Computational Biology General Medicine medicine.disease Leukemia Lymphoid Vaccination medicine.anatomical_structure Immunology biology.protein Antibody Multiple Myeloma Peptides CD8 T-Lymphocytes Cytotoxic |
| Popis: | Although the idiotypic structures of immunoglobulin from malignant B cells were the first tumor-specific determinants recognized, and clinical vaccination trials have demonstrated induction of tumor-specific immunity, the function of immunoglobulin-specific CD8+ cytotoxic T lymphocytes in tumor rejection remains elusive. Here, we combined bioinformatics and a T cell-expansion system to identify human immunoglobulin-derived peptides capable of inducing cytotoxic T-lymphocyte responses. Immunogenic peptides were derived from framework regions of the variable regions of the immunoglobulin that were shared among patients. Human-leukocyte-antigen-matched and autologous cytotoxic T lymphocytes specific for these peptides killed primary malignant B cells, demonstrating that malignant B cells are capable of processing and presenting such peptides. Targeting shared peptides to induce T-cell responses might further improve current vaccination strategies in B-cell malignancies. |
| Databáze: | OpenAIRE |
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