Upregulation of FosB/ΔFosB in limbic circuits after tooth exodontia-induced occlusal instability in an experimental model of unpredictable chronic stress
| Autor: | Mariza Bortolanza, A.C. Issy, João Francisco Cordeiro Pedrazzi, Elaine Del-Bel, Daniele P. Ferrari, Yamba Carla Lara Pereira, Christie Ramos Andrade Leite-Panissi, Daniela Mizusaki Iyomasa, João Paulo Mardegan Issa, Bruna Balbino de Paula, Mamie Mizusaki Iyomasa, Glauce Crivelaro do Nascimento |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Elevated plus maze Nucleus accumbens Limbic system Internal medicine medicine Limbic System Animals Chronic stress Rats Wistar Neurons Diazepam business.industry Dental occlusion General Neuroscience Central nucleus of the amygdala Immunohistochemistry Rats Up-Regulation Endocrinology medicine.anatomical_structure Anti-Anxiety Agents Hypothalamus Tooth Extraction business Proto-Oncogene Proteins c-fos Stress Psychological FOSB |
| Zdroj: | Brain research bulletin. 176 |
| ISSN: | 1873-2747 |
| Popis: | Psychological stress and occlusal alterations are contributing etiologic factors for temporomandibular and muscular disorders in the orofacial area. The neural modulation recruited for this relationship, however, is not elucidated. The aim of this study was to investigate potential central mechanisms involved in the exodontia-induced occlusal instability associated with unpredictable chronic stress (UCS). Male adult Wistar rats were submitted to occlusal instability (unilateral molar teeth extraction) and/or to a UCS protocol and treated with diazepam or vehicle. The anxiety-like behavior was evaluated by elevated plus maze (EPM) and open field (OF) tests. Limbic structures such as the central nucleus of the amygdala (CeA), paraventricular nucleus of the hypothalamus (PVN), dorsal periaqueductal gray matter (dPAG) and nucleus accumbens core (NAc) were analyzed for expression of FosB/ΔFosB (immediate early genes) by immunohistochemistry. Exodontia and/or UCS decreased the time spent in the open arms at the EPM and the distance travelled at the OF, and increased the immobility time at the OF, suggesting anxiety-like behavior. In addition, exodontia induction resulted in an upregulation of FosB/ΔFosB in the CeA, PVN and dPAG, while UCS and exodontia + UCS upregulate FosB/ΔFosB immunoreactivity in the CeA, PVN, dPAG and NAc. Treatment with diazepam decreased the expression of FosB/ΔFosB in all analyzed structures of animals subject to UCS and exodontia + UCS, while promoted a reduction in the FosB/ΔFosB expression in the CeA, PVN and dPAG in animals subject to exodontia. Our findings showed an anxiogenic effect of exodontia and UCS, which is correlated with intranuclear neuron activation of limbic structures in a spatially dependent manner and that is prevented by the administration of diazepam. |
| Databáze: | OpenAIRE |
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