Endoplasmic reticulum stress is involved in lipopolysaccharide‐induced inflammatory response and apoptosis in goat endometrial stromal cells
| Autor: | Diqi Yang, Amira Abdalla Abdelshafy Mohamed, Shouqin Liu, Pengfei Lin, Osama Abdalla Abdelshafy Mohamad, Yaping Jin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Cell Survival medicine.medical_treatment Apoptosis Protein Serine-Threonine Kinases Biology Transfection Proinflammatory cytokine Endometrium 03 medical and health sciences 0302 clinical medicine Endoribonucleases Genetics medicine Animals Secretion Cells Cultured Cell Proliferation Endometrial Stromal Cell 030219 obstetrics & reproductive medicine Goats Endoplasmic reticulum Cell Biology Endoplasmic Reticulum Stress Phenylbutyrates Activating Transcription Factor 6 Cell biology 030104 developmental biology Cytokine Gene Knockdown Techniques Unfolded Protein Response Unfolded protein response Cytokines Female Cytokine secretion Stromal Cells Endometritis Signal Transduction Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 86:908-921 |
| ISSN: | 1098-2795 1040-452X |
| Popis: | Endoplasmic reticulum (ER) stress is involved in regulating cell metabolism, apoptosis, autophagy, and survival. However, there is not enough information about the role of ER stress in lipopolysaccharide (LPS)-induced apoptosis and inflammatory cytokine secretion in the uterus. In this study, we found that LPS induced apoptosis and inflammation in goat endometrial stromal cells (ESCs). LPS treatment inhibited cell viability and cell proliferation. In addition, the genes associated with proliferation, such as proliferating cell nuclear antigen and MKI67, were affected by LPS treatment. Moreover, LPS increased the secretion of interleukin (IL)-1β and IL-8, promoting the levels of MYD88, caspase1, and TRL4. The 4-phenylbutyric acid pretreatment inhibited the expression of unfolded protein response proteins and the secretion of inflammatory cytokines in LPS-treated cells. However, blockage of inositol-requiring enzyme 1 and activating transcription factor 6 did not significantly reduce apoptosis and inflammatory cytokine secretion. Collectively, ER stress involved in LPS-induced apoptosis and inflammatory cytokine increased in goat ESCs. This study provides new insight into the function of ER stress in the pathological process. |
| Databáze: | OpenAIRE |
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