Quantitative determination of small selenium species in human serum by HPLC/ICPMS following a protein-removal, pre-concentration procedure
| Autor: | Doris Kuehnelt, Ulrike Hartleb, Sabine Kokarnig, Michael Stiboller, Kevin A. Francesconi |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Spectrometry Mass Electrospray Ionization Chromatography chemistry.chemical_element Proteins Biochemistry Selenate High-performance liquid chromatography Analytical Chemistry Matrix (chemical analysis) Methylselenocysteine chemistry.chemical_compound Selenium chemistry Organoselenium Compounds Dietary Supplements Protein precipitation Humans Sample preparation Female Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Analytical and bioanalytical chemistry. 400(8) |
| ISSN: | 1618-2650 |
| Popis: | Protein precipitation was incorporated into a sample preparation method for the quantitative determination of small “non-protein” selenium species in human serum by high-performance liquid chromatography–inductively coupled plasma mass spectrometry (HPLC/ICPMS). The advantages of cleaner matrix and concomitant concentration of the small compounds result in quantification limits in the native serum at the sub-micrograms Se per litre level. Spiking experiments with methyl 2-acetamido-2-deoxy-1-seleno-β-d-galactopyranoside (selenosugar 1), trimethylselenonium ion, selenomethionine, methylselenocysteine (MeSeCys) and selenate yielded recoveries from 73% to 103%. Selenite had a low recovery (44%), possibly owing to protein binding. The validated method was applied to serum samples from two volunteers before and after ingestion of a selenium food supplement. HPLC/ICPMS analysis showed, besides ingested selenate, the presence of selenosugar 1 and trace amounts of MeSeCys and methyl 2-amino-2-deoxy-1-seleno-β-d-galactopyranoside, which have not been reported in human serum before. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|