Manipulating human dendritic cell phenotype and function with targeted porous silicon nanoparticles
| Autor: | Shilpanjali Jesudason, Robert P. Carroll, Peter D. Rose, Jean Durand, Darling Rojas-Canales, Sebastian O. Stead, Steven J. P. McInnes, David Warther, Nicolas H. Voelcker, Svjetlana Kireta, Frédérique Cunin, P. Toby Coates, Chris Drogemuller |
|---|---|
| Přispěvatelé: | Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Stead, Sebastian O, McInnes, Steven JP, Kireta, Svjetlana, Rose, Peter D, Jesudason, Shilpanjali, Rojas-Canales, Darling, Warther, David, Cunin, Frédérique, Durand, Jean-Olivier, Drogemuller, Christopher J, Carroll, Robert P, Coates, P Toby, Voelcker, Nicolas H |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Silicon Materials science [SDV]Life Sciences [q-bio] Biophysics Nanoparticle Bioengineering 02 engineering and technology [CHIM.INOR]Chemical Sciences/Inorganic chemistry immunomodulation Dendritic cells Biomaterials Immunomodulation 03 medical and health sciences Porous silicon medicine Humans [CHIM]Chemical Sciences Rapamycin Receptor targeting Targeting rapamycin Adhesion Dendritic cell [CHIM.MATE]Chemical Sciences/Material chemistry 021001 nanoscience & nanotechnology nanomedicine Phenotype Cell biology porous silicon 030104 developmental biology Nanomedicine Mechanics of Materials Sirolimus Immunology Ceramics and Composites Nanoparticles nanoparticles 0210 nano-technology Porosity Intracellular medicine.drug |
| Zdroj: | Biomaterials Biomaterials, Elsevier, 2018, 155, pp.92-102. ⟨10.1016/j.biomaterials.2017.11.017⟩ |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2017.11.017⟩ |
| Popis: | International audience; Dendritic cells (DC) are the most potent antigen-presenting cells and are fundamental for the establishment of transplant tolerance. The Dendritic Cell-Specific Intracellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN; CD209) receptor provides a target for dendritic cell therapy. Biodegradable and high-surface area porous silicon (pSi) nanoparticles displaying anti-DC-SIGN antibodies and loaded with the immunosuppressant rapamycin (Sirolimus) serve as a fit-for-purpose platform to target and modify DC. Here, we describe the fabrication of rapamycin-loaded DC-SIGN displaying pSi nanoparticles, the uptake efficiency into DC and the extent of nanoparticle-induced modulation of phenotype and function. DC-SIGN antibody displaying pSi nanoparticles favourably targeted and were phagocytosed by monocyte-derived and myeloid DC in whole human blood in a time- and dose-dependent manner. DC preconditioning with rapamycin-loaded nanoparticles, resulted in a maturation resistant phenotype and significantly suppressed allogeneic T-cell proliferation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect