Psoriasin has divergent effects on the innate immune responses of murine glial cells
| Autor: | Ronald Wolf, Sandra Jansen, Eugenia Kress, Thomas Pufe, Christoph Jan Wruck, Matthias Michalek, Athanassios Fragoulis, Simone C. Tauber, Joachim Grötzinger, Lars-Ove Brandenburg |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine S100A7 Biology Biochemistry S100 Calcium Binding Protein A7 Mice 03 medical and health sciences Cellular and Molecular Neuroscience Immune system medicine Animals Humans Transcription factor Cells Cultured Neuroinflammation Innate immune system Microglia S100 Proteins Immunity Innate Cell biology Mice Inbred C57BL HEK293 Cells 030104 developmental biology medicine.anatomical_structure Animals Newborn Female Tumor necrosis factor alpha Inflammation Mediators Signal transduction Neuroglia |
| Zdroj: | Journal of Neurochemistry. 141:86-99 |
| ISSN: | 0022-3042 |
| Popis: | Antimicrobial peptides (AMP) are an important part of the innate immune defense in the central nervous system (CNS). The expression of the AMP psoriasin (S100A7) is up-regulated during bacterial meningitis. However, the exact mechanisms induced by psoriasin to modulate glial cell activity are not yet fully understood. Our hypothesis is that psoriasin induced pro and anti-inflammatory signaling pathways as well as regenerative factors to contribute in total to a balanced immune response. Therefore, we used psoriasin stimulated glial cells and analyzed the translocation of the pro-inflammatory transcription factor nuclear factor ‘kappa-light-chain-enhancer’ of activated B-cells (NFκB) in murine glial cells and the expression of pro- and anti-inflammatory mediators by real time RT-PCR, ELISA technique and Western blotting. Furthermore, the relationship between psoriasin and the anti-oxidative stress transcription factor nuclear factor erythroid 2 related factor 2 (Nrf2) was investigated. Stimulation with psoriasin enhanced NFκB translocation and increased the expression of the pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF- α) but also neurotrophin expression. Evidence for functional interactions between psoriasin and Nrf2 were detected in form of increased antioxidant response element (ARE) activity and induction of Nrf2/ARE-dependent heme oxygenase 1 (HO-1) expression in psoriasin-treated microglia and astrocytes. The results illustrate the ability of psoriasin to induce immunological functions in glia cells where psoriasin exerts divergent effects on the innate immune response. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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