Aesculus hippocastanum-Derived Extract β-Aescin and In vitro Antibacterial Activity
| Autor: | Salma L Dahash, Hayder M Al-Kuraishy, Ourba K Abass, Ali I Al-Gareeb, Myada M Abdul-Razaq |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Aescin
Minimum bactericidal concentration biology Serial dilution Klebsiella pneumoniae Chemistry minimal inhibitory concentration medicine.disease_cause biology.organism_classification Pathology and Forensic Medicine Electronic Optical and Magnetic Materials Microbiology chemistry.chemical_compound Minimum inhibitory concentration β-aescin Agar well diffusion antibacterial activity Nephrology Staphylococcus aureus Staphylococcus epidermidis minimal bactericidal concentration medicine Original Article Antibacterial activity Instrumentation |
| Zdroj: | Journal of Microscopy and Ultrastructure |
| ISSN: | 2213-8803 2213-879X |
| Popis: | Objectives: The objective of this study was to investigate the antibacterial activity of β-aescin against common Gram-negative and Gram-positive bacteria. Materials and Methods: Agar well diffusion assay was used to determine the antibacterial activity of β-aescin against common Gram-negative and Gram-positive bacteria including Klebsiella pneumoniae, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of β-aescin were evaluated by serial dilution method. Results: β-aescin led to significant antibacterial effects on the tested Gram-negative and Gram-positive bacteria compared to the negative control, P < 0.05 for K. pneumoniae and P. aeruginosa and P < 0.01 for E. coli, S. epidermidis, and S. aureus. On the other hand, β-aescin produced a comparable less antibacterial effect on K. pneumoniae, E. coli, and P. aeruginosa compared to the positive control, P < 0.01, whereas β-aescin illustrated a comparable effect with that of the positive control on Gram-positive S. epidermidis, P = 0.05. Furthermore, β-aescin illustrated a concentration-dependent antibacterial effect against Gram-positive S. epidermidis and S. aureus compared to the different concentrations, P < 0.01. MIC and MBC of β-aescin were high for Gram-negative bacteria and low for Gram-positive bacteria compared to MIC of the positive control. Conclusions: β-aescin is an effective antibacterial herb mainly against Gram-positive S. epidermidis and S. aureus in a concentration-dependent manner. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|