Combined therapy of the antimetastatic compound NAMI-A and electroporation on B16F1 tumour cells in vitro
| Autor: | Damijan Miklavčič, Iztok Turel, Maša Kandušer, Ajda Bicek |
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| Rok vydání: | 2007 |
| Předmět: |
Electrochemotherapy
Cell Survival Biophysics Melanoma Experimental Antineoplastic Agents Biology chemistry.chemical_compound Mice Cell Line Tumor Electrochemistry medicine Organometallic Compounds NAMI-A Animals Dimethyl Sulfoxide Viability assay Physical and Theoretical Chemistry Neoplasm Metastasis Clonogenic assay Electroporation Melanoma General Medicine medicine.disease Molecular biology In vitro chemistry Cell culture Cancer research Ruthenium Compounds |
| Zdroj: | Bioelectrochemistry (Amsterdam, Netherlands). 71(2) |
| ISSN: | 1567-5394 |
| Popis: | Ruthenium complex NAMI-A [ImH][trans-RuCl(4)(DMSO-S)Im] (Im = imidazole) is a potential chemotherapeutic drug in cancer treatment. Electroporation can be used to facilitate delivery of NAMI-A into cells. Suspension of B16F1 tumour cells from mouse melanoma in NAMI-A solution was exposed to a train of electric pulses. The effect of NAMI-A was determined by examining cell viability in clonogenic test. Our results show that electroporation increases the otherwise scarce in vitro effects of NAMI-A, i.e. reduces cell viability. At the conditions chosen for experiments 90% of cells survived in the presence of 1 microM NAMI-A, whereas in a combined treatment with 1 microM NAMI-A and electroporation only about 10% of cells survived. |
| Databáze: | OpenAIRE |
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