Diagnosis of group A coxsackieviral infection using polymerase chain reaction
| Autor: | Hitoshi Suzuki, Y Shimada, K Honzumi, S Suzuki, H Ishiko, Mitsuaki Hosoya, K Kato |
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| Rok vydání: | 2002 |
| Předmět: |
Herpangina
Fever Tonsillitis Genome Viral Coxsackievirus medicine.disease_cause Polymerase Chain Reaction Virus Seizures Febrile law.invention law Culture Techniques Virology medicine Enterovirus Infections Humans Child Polymerase chain reaction Phylogeny biology business.industry Infant Pharyngitis medicine.disease biology.organism_classification Enterovirus A Human Child Preschool Pediatrics Perinatology and Child Health Acute Disease Enterovirus Pharynx Original Article Viral disease medicine.symptom business |
| Zdroj: | Archives of disease in childhood. 87(4) |
| ISSN: | 1468-2044 |
| Popis: | Aims: To examine the relation between enteroviral infection, especially group A coxsackieviral infection, and acute febrile illness over two summers using tissue culture and polymerase chain reaction (PCR). Methods: Throat swabs were collected from 246 children from June to August 1997 and 1998. Results: Enteroviruses were isolated from 33/246 samples and 35 other viruses were isolated. Enteroviral genomes were detected in 54/178 samples from which no virus was isolated. Of 41 enteroviral genotypes identified by sequence analysis of PCR products, 38 were group A coxsackieviruses, which are usually difficult to isolate using tissue culture. Conclusion: Results indicate that viral detection and identification based on PCR is useful in the diagnosis of group A coxsackieviral infection. |
| Databáze: | OpenAIRE |
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