Interdomain communication of the Chd1 chromatin remodeler across the DNA gyres of the nucleosome
| Autor: | Ashok Kumar Patel, Gregory D. Bowman, Ren Ren, Ilana M. Nodelman, Kyle Horvath, Franziska Bleichert, James M. Berger |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Nucleosome assembly Context (language use) Cell Biology Solenoid (DNA) DNA Biology Chromatin Assembly and Disassembly Linker DNA SWI/SNF Article Chromatin Cell biology Nucleosomes DNA-Binding Proteins 03 medical and health sciences Xenopus laevis 030104 developmental biology Protein Domains Chromatosome Nucleosome Animals Molecular Biology Protein Binding |
| Popis: | Chromatin remodelers use a helicase-like ATPase motor to reposition and reorganize nucleosomes along genomic DNA. Yet, how the ATPase motor communicates with other remodeler domains in the context of the nucleosome has so far been elusive. Here, we report for the Chd1 remodeler a unique organization of domains on the nucleosome that reveals direct domain-domain communication. Site-specific cross-linking shows that the chromodomains and ATPase motor bind to adjacent SHL1 and SHL2 sites, respectively, on nucleosomal DNA and pack against the DNA-binding domain on DNA exiting the nucleosome. This domain arrangement spans the two DNA gyres of the nucleosome and bridges both ends of a wrapped, ∼90-bp nucleosomal loop of DNA, suggesting a means for nucleosome assembly. This architecture illustrates how Chd1 senses DNA outside the nucleosome core and provides a basis for nucleosome spacing and directional sliding away from transcription factor barriers. |
| Databáze: | OpenAIRE |
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