The investigation of calpain in human placenta with fetal growth restriction
| Autor: | Eita Mizutani, Yasuhiko Ozaki, Tadahide Furuno, Tamao Kitaori, Nobuhiro Suzumori, Mayumi Sugiura-Ogasawara, Klas Blomgren, Shinobu Goto, Fumiko Ozawa |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
0301 basic medicine Placenta Immunology Biology Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy medicine Humans Immunology and Allergy reproductive and urinary physiology Calpastatin Fetal Growth Retardation 030219 obstetrics & reproductive medicine Calpain Calcium-Binding Proteins Obstetrics and Gynecology Trophoblast Staining Blot 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Cytoplasm embryonic structures biology.protein Immunohistochemistry Female |
| Zdroj: | American Journal of Reproductive Immunology. 85 |
| ISSN: | 1600-0897 1046-7408 |
| DOI: | 10.1111/aji.13325 |
| Popis: | PROBLEM The mechanism of fetal growth restriction (FGR) is not fully understood. In this study, we explored the contribution of the calpain-calpastatin system and the activated states of calpains in human FGR placenta. METHOD OF STUDY The placentas were collected from patients of FGR (n = 17) and controls (n = 23) at elective cesarean sections in Nagoya City University Hospital and used for experiments upon informed consent. The existence and the expression of calpains and calpastatin in human placenta were compared between FGR and controls using immunohistochemistry, SDS-PAGE, and Western blotting. RESULTS Staining of calpains (pre-, post-μ-calpain, pre-, post-m-calpain, and calpain-6) and calpastatin was observed in cytoplasm of trophoblast cells, both in FGR and control placenta. Pre-μ-calpain was located in the cytoplasm, and post-μ-calpain was located mainly in proximity to the cytoplasmic membrane. The expression of pre-μ-calpain was significantly higher (P |
| Databáze: | OpenAIRE |
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