Biochemical study of idebenone effect on mitochondrial metabolism of yeast
| Autor: | Inés Nievas, Hilda I. Burgos, Carlos A. Stella, Sebastián Pablo Chapela, Ana Inés Salazar, Norberto Kriguer |
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| Rok vydání: | 2007 |
| Předmět: |
Membrane potential
biology Ubiquinone Saccharomyces cerevisiae Mutant Cell Cell Biology General Medicine Metabolism biology.organism_classification Rhodamine 123 Yeast Culture Media Mitochondria Oxygen chemistry.chemical_compound medicine.anatomical_structure Biochemistry chemistry medicine Idebenone medicine.drug |
| Zdroj: | Cell biology international. 32(1) |
| ISSN: | 1065-6995 |
| Popis: | The aim of this work was to study the effect of the drug idebenone on the growth of a strain of Saccharomyces cerevisiae yeast and its respiratory-deficient mutant (rho0). We took this yeast as a model system of the interaction of the drug with mammalian cells. The effect of idebenone was evaluated in rich and minimal media. In the S288c strain, idebenone exerted a growth inhibitory effect in concentrations higher than 50 μM in media containing a carbon source consumed at mitochondrial level. In conditions of low oxygen supply, idebenone allows yeast to keep a cellular yielding comparable with conditions of normal oxygen supply. Also, the presence of idebenone in the growth media increased by 50% the fluorescence signal of rhodamine 123, indicating a higher mitochondrial membrane potential. The results could explain the effect of idebenone in the treatment of diseases in which oxygen deficiency alters the energetic metabolism of the cell. |
| Databáze: | OpenAIRE |
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