Protective effects of Radix Codonopsis on ischemia-reperfusion injury in rats after kidney transplantation

Autor: Tao Lin, Guanghui Wei, Xingang Yuan, Bo He, Jing-Song Sun, Ying-Tian Zhang
Rok vydání: 2011
Předmět:
Zdroj: Pediatric surgery international. 27(11)
ISSN: 1437-9813
Popis: Kidney ischemia–reperfusion injury (IRI) after kidney transplant remains a major problem, separate from immune rejection that can lead to kidney transplant failure and graft function loss. Free radicals, disturbance of microcirculation and the inflammatory cascade appear to be the main contributors. Radix Codonopsis, a traditional Chinese drug used in vascular diseases, is an antioxidant and free radical scavenger. This study investigates the protective effect and underlying mechanisms of Radix Codonopsis extract saponins on kidney transplantation. Renal transplantation was performed after rat kidneys had been stored for 1 h at 4°C. Blood urea nitrogen (BUN), serum creatinine (Scr), superoxide dismutase (SOD) and malondialdehyde (MDA) were assayed; bcl-2 and bax mRNA expression was detected using RT-PCR; bcl-2 and bax protein expression was detected by immunohistochemistry (IHC). Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) was used to detect apoptotic cells and determine the apoptosis index (AI). Analysis of variance (ANOVA) followed by Dunnett’s test was used when more than two groups were compared. Saponin-treated animals showed increased SOD levels accompanied by decreased MDA, Scr and BUN levels (p
Databáze: OpenAIRE