Protective effects of Radix Codonopsis on ischemia-reperfusion injury in rats after kidney transplantation
Autor: | Tao Lin, Guanghui Wei, Xingang Yuan, Bo He, Jing-Song Sun, Ying-Tian Zhang |
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Rok vydání: | 2011 |
Předmět: |
Graft Rejection
Male Apoptosis Andrology Rats Sprague-Dawley Malondialdehyde medicine In Situ Nick-End Labeling Animals RNA Messenger Blood urea nitrogen Codonopsis Kidney transplantation bcl-2-Associated X Protein Kidney TUNEL assay biology business.industry Plant Extracts Reverse Transcriptase Polymerase Chain Reaction Superoxide Dismutase General Medicine Free Radical Scavengers medicine.disease biology.organism_classification Free radical scavenger Immunohistochemistry Kidney Transplantation Rats Transplantation Disease Models Animal Oxidative Stress medicine.anatomical_structure Treatment Outcome Proto-Oncogene Proteins c-bcl-2 Reperfusion Injury Pediatrics Perinatology and Child Health Immunology Surgery business Reperfusion injury |
Zdroj: | Pediatric surgery international. 27(11) |
ISSN: | 1437-9813 |
Popis: | Kidney ischemia–reperfusion injury (IRI) after kidney transplant remains a major problem, separate from immune rejection that can lead to kidney transplant failure and graft function loss. Free radicals, disturbance of microcirculation and the inflammatory cascade appear to be the main contributors. Radix Codonopsis, a traditional Chinese drug used in vascular diseases, is an antioxidant and free radical scavenger. This study investigates the protective effect and underlying mechanisms of Radix Codonopsis extract saponins on kidney transplantation. Renal transplantation was performed after rat kidneys had been stored for 1 h at 4°C. Blood urea nitrogen (BUN), serum creatinine (Scr), superoxide dismutase (SOD) and malondialdehyde (MDA) were assayed; bcl-2 and bax mRNA expression was detected using RT-PCR; bcl-2 and bax protein expression was detected by immunohistochemistry (IHC). Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) was used to detect apoptotic cells and determine the apoptosis index (AI). Analysis of variance (ANOVA) followed by Dunnett’s test was used when more than two groups were compared. Saponin-treated animals showed increased SOD levels accompanied by decreased MDA, Scr and BUN levels (p |
Databáze: | OpenAIRE |
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