Comprehensive Proteomic and Metabolomic Signatures of Nontypeable Haemophilus influenzae-Induced Acute Otitis Media Reveal Bacterial Aerobic Respiration in an Immunosuppressed Environment
| Autor: | Derek R. Heimlich, Rachael L. Hardison, Laura G. Dubois, M. Arthur Moseley, Kevin M. Mason, Alistair Harrison, J. Will Thompson, Sheryl S. Justice, Lisa St. John-Williams, Joseph E. Kerschner, Alexander Stoddard |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Proteomics Haemophilus Infections Cellular respiration 030106 microbiology Cell medicine.disease_cause Biochemistry Actin-Related Protein 2-3 Complex Analytical Chemistry Haemophilus influenzae Microbiology Pathogenesis 03 medical and health sciences Metabolomics In vivo Chinchilla Tandem Mass Spectrometry medicine Animals Humans Molecular Biology biology biology.organism_classification Disease Models Animal Otitis Media 030104 developmental biology Otitis medicine.anatomical_structure Host-Pathogen Interactions medicine.symptom Bacteria Chromatography Liquid Regular Articles |
| Zdroj: | Molecularcellular proteomics : MCP. 15(3) |
| ISSN: | 1535-9484 |
| Popis: | A thorough understanding of the molecular details of the interactions between bacteria and host are critical to ultimately prevent disease. Recent technological advances allow simultaneous analysis of host and bacterial protein and metabolic profiles from a single small tissue sample to provide insight into pathogenesis. We used the chinchilla model of human otitis media to determine, for the first time, the most expansive delineation of global changes in protein and metabolite profiles during an experimentally induced disease. After 48 h of infection with nontypeable Haemophilus influenzae, middle ear tissue lysates were analyzed by high-resolution quantitative two-dimensional liquid chromatography-tandem mass spectrometry. Dynamic changes in 105 chinchilla proteins and 66 metabolites define the early proteomic and metabolomic signature of otitis media. Our studies indicate that establishment of disease coincides with actin morphogenesis, suppression of inflammatory mediators, and bacterial aerobic respiration. We validated the observed increase in the actin-remodeling complex, Arp2/3, and experimentally showed a role for Arp2/3 in nontypeable Haemophilus influenzae invasion. Direct inhibition of actin branch morphology altered bacterial invasion into host epithelial cells, and is supportive of our efforts to use the information gathered to modify outcomes of disease. The twenty-eight nontypeable Haemophilus influenzae proteins identified participate in carbohydrate and amino acid metabolism, redox homeostasis, and include cell wall-associated metabolic proteins. Quantitative characterization of the molecular signatures of infection will redefine our understanding of host response driven developmental changes during pathogenesis. These data represent the first comprehensive study of host protein and metabolite profiles in vivo in response to infection and show the feasibility of extensive characterization of host protein profiles during disease. Identification of novel protein targets and metabolic biomarkers will advance development of therapeutic and diagnostic options for treatment of disease. |
| Databáze: | OpenAIRE |
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