Ibuprofen and indomethacin differentially regulate vascular endothelial growth factor and its receptors in ductus arteriosus endothelial cells
| Autor: | Sven C. Weber, Petra Koehne, Alexander Gratopp, Hannes Sallmon, C. Rheinländer, Sandra Akanbi |
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| Rok vydání: | 2017 |
| Předmět: |
Indomethacin
Cell Culture Techniques Fluorescent Antibody Technique Ibuprofen 030204 cardiovascular system & hematology Pharmacology 03 medical and health sciences chemistry.chemical_compound Fetus 0302 clinical medicine 030225 pediatrics medicine.artery Ductus arteriosus Animals Medicine Cyclooxygenase Inhibitors RNA Messenger Rats Wistar Receptor Aorta biology Vascular Endothelial Growth Factors Ductus arteriosus closure business.industry Endothelial Cells Kinase insert domain receptor Ductus Arteriosus General Medicine Rats Endothelial stem cell Vascular endothelial growth factor medicine.anatomical_structure chemistry Pediatrics Perinatology and Child Health biology.protein Female Cyclooxygenase Cardiology and Cardiovascular Medicine business |
| Zdroj: | Cardiology in the Young. 28:432-437 |
| ISSN: | 1467-1107 1047-9511 |
| DOI: | 10.1017/s1047951117002311 |
| Popis: | BackgroundCyclooxygenase inhibitors are widely applied to facilitate ductal closure in preterm infants. The mechanisms that lead to patent ductus arteriosus closure are incompletely understood. Vascular endothelial growth factor plays pivotal roles during ductal closure and remodelling.AimThe aim of this study was to investigate the effects of ibuprofen and indomethacin on the expression of vascular endothelial growth factor and its receptors in a primary rat ductus arteriosus endothelial cell culture.MethodsProtein expression of vascular endothelial growth factor and vascular endothelial growth factor receptor 1 and 2 was confirmed in rat ductus arteriosus and aorta by immunofluorescence staining. Fetal rat endothelial cells were isolated from ductus arteriosus and aorta using immunomagnetic cell sorting and treated with ibuprofen or indomethacin. mRNA expression levels were assessed by quantitative polymerase chain reaction analysis.ResultsIn ductal endothelial cells, ibuprofen significantly induced vascular endothelial growth factor and its receptor 2, but not receptor 1, whereas indomethacin did not alter the expression levels of the vascular endothelial growth factor system. In contrast, ibuprofen significantly induced vascular endothelial growth factor and its receptors 1 and 2 in aortic endothelial cells, whereas indomethacin only induced vascular endothelial growth factor receptor 2.ConclusionOur results indicate differential effects of ibuprofen and indomethacin on the expression levels of the vascular endothelial growth factor system in ductus arteriosus endothelial cells. In addition, vessel-specific differences between ductal and aortic endothelial cells were found. Further in vivo studies are needed to elucidate the biological significance of these findings. |
| Databáze: | OpenAIRE |
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