Myristoleic Acid Promotes Anagen Signaling by Autophagy through Activating Wnt/β-Catenin and ERK Pathways in Dermal Papilla Cells
| Autor: | Ji-Hoon Kang, Youn Kyung Choi, Jin Won Hyun, Chang-Gu Hyun, Jung-Il Kang, Kwang-Sik Lee, Hee-Kyoung Kang, Tae Yang Kim, Young Sang Koh, Chun Mong Lee, Kyung-Sup Yoon, Eun-Sook Yoo |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Cyclin A Dermal papilla cells Biochemistry 03 medical and health sciences 0302 clinical medicine Hair cycle Drug Discovery Autophagy Anagen Cyclin B1 Pharmacology Wnt/β-catenin Cyclin-dependent kinase 1 integumentary system biology Chemistry Wnt signaling pathway Cell biology ERK 030104 developmental biology 030220 oncology & carcinogenesis Catenin biology.protein Molecular Medicine Original Article Myristoleic acid |
| Zdroj: | Biomolecules & Therapeutics |
| ISSN: | 2005-4483 1976-9148 |
| DOI: | 10.4062/biomolther.2020.169 |
| Popis: | Alopecia is a distressing condition caused by the dysregulation of anagen, catagen, and telogen in the hair cycle. Dermal papilla cells (DPCs) regulate the hair cycle and play important roles in hair growth and regeneration. Myristoleic acid (MA) increases Wnt reporter activity in DPCs. However, the action mechanisms of MA on the stimulation of anagen signaling in DPCs is not known. In this study, we evaluated the effects of MA on anagen-activating signaling pathways in DPCs. MA significantly increased DPC proliferation and stimulated the G2/M phase, accompanied by increasing cyclin A, Cdc2, and cyclin B1. To elucidate the mechanism by which MA promotes DPC proliferation, we evaluated the effect of MA on autophagy and intracellular pathways. MA induced autophagosome formation by decreasing the levels of the phospho-mammalian target of rapamycin (phospho-mTOR) and increasing autophagy-related 7 (Atg7) and microtubule-associated protein 1A/1B-light chain 3II (LC3II). MA also increased the phosphorylation levels of Wnt/β-catenin proteins, such as GSK3β (Ser9) and β-catenin (Ser552 and Ser675). Treatment with XAV939, an inhibitor of the Wnt/β-catenin pathway, attenuated the MA-induced increase in β-catenin nuclear translocation. Moreover, XAV939 reduced MA-induced effects on cell cycle progression, autophagy, and DPC proliferation. On the other hand, MA increased the levels of phospho (Thr202/Tyr204)-extracellular signal regulated kinases (ERK). MA-induced ERK phosphorylation led to changes in the expression levels of Cdc2, Atg7 and LC3II, as well as DPC proliferation. Our results suggest that MA promotes anagen signaling via autophagy and cell cycle progression by activating the Wnt/β-catenin and ERK pathways in DPCs. |
| Databáze: | OpenAIRE |
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