Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension

Autor: Mina Toljic, Jovana Vuceljic, Amira Egic, Ivana Joksic, Zagorka Milovanovic, Natasa Karadzov Orlic, Jelena Munjas, Aleksandra Radenkovic
Rok vydání: 2017
Předmět:
Adult
0301 basic medicine
medicine.medical_specialty
endocrine system diseases
Micronuclei
Toxicology
medicine.disease_cause
Gestational diabetes mellitus
Thiobarbituric Acid Reactive Substances
Young Adult
03 medical and health sciences
chemistry.chemical_compound
Pregnancy
Malondialdehyde
Internal medicine
TBARS
Humans
Medicine
Gestational arterial hypertension
Lymphocytes
Methyldopa
Micronuclei
Chromosome-Defective
Cytokinesis
Micronucleus Tests
business.industry
Deoxyguanosine
Hypertension
Pregnancy-Induced
medicine.disease
8-Hydroxy-2'-deoxyguanosine
3. Good health
Gestational diabetes
Diabetes
Gestational
Oxidative Stress
030104 developmental biology
Endocrinology
chemistry
Oxidative stress
8-Hydroxy-2'-Deoxyguanosine
Micronucleus test
Female
Cytokinesis-block micronucleus cytome assay
business
Micronucleus
DNA Damage
medicine.drug
Zdroj: Research in Microbiology
ISSN: 0890-6238
DOI: 10.1016/j.reprotox.2017.04.002
Popis: We investigated whether gestational diabetes mellitus (GDM) and gestational arterial hypertension (GH) are associated with increased oxidative stress and DNA damage. Study included 3 groups of pregnant women (GDM, GH and control). DNA damage biomarkers (micronuclei MNi, nucleoplasmic bridges NPBs and nuclear buds NBUDs) were assessed by cytokinesis-block micronucleus cytome assay. Oxidative stress levels were evaluated by analyzing malondialdehyde equivalents (TBARS) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Genotoxic effect of methyldopa, drug used to treat GH, was evaluated in in vitro experiment. TBARS levels, MNi, NPBs and NBUDs frequencies were significantly increased in both GDM and GH group. Concentrations of 8-OHdG were significantly higher in GDM than in other groups. Since methyldopa did not affect MNi, NPBs and NBUDs frequencies, nor TBARS and 8-OHdG levels, we concluded that methyldopa has no genotoxic effect. Thus, even when hyperglycemia or hypertension are present only during pregnancy they induce oxidative stress, DNA damage and chromosomal aberrations.
Databáze: OpenAIRE
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