Priming by microbial antigens from the intestinal flora determines the ability of CD4+ T cells to rapidly secrete IL-4 in BALB/c mice infected with Leishmania major
| Autor: | Alain Beck, Fernand Girard-Pipau, Stephen S. McSorley, Valérie Julia, Laurent Malherbe, Nicolas Glaichenhaus, Jean-Philippe Breittmayer |
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| Rok vydání: | 2000 |
| Předmět: |
CD4-Positive T-Lymphocytes
Immunology Molecular Sequence Data Priming (immunology) Epitopes T-Lymphocyte Leishmaniasis Cutaneous Mice Nude Mice Transgenic Biology Lymphocyte Activation Microbiology Interleukin 21 Mice Immune system Antigen Bacterial Proteins Enterococcus faecalis Escherichia coli Immunology and Allergy Animals Germ-Free Life IL-2 receptor Amino Acid Sequence RNA Messenger Intestinal Mucosa Antigen-presenting cell Interleukin 4 Interleukin 3 Leishmania major Antigens Bacterial Mice Inbred BALB C Hybridomas Antibodies Monoclonal Cell Differentiation Anti-Bacterial Agents ATP-Binding Cassette Transporters Female Disease Susceptibility Interleukin-4 Injections Intraperitoneal |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 165(10) |
| ISSN: | 0022-1767 |
| Popis: | Infection of BALB/c mice with Leishmania major results in the rapid accumulation of IL-4 transcripts within CD4+ T cells that react to the parasite Leishmania homologue of mammalian RACK1 (LACK) Ag. Because memory/effector cells secrete IL-4 more rapidly than naive cells, we sought to analyze the phenotype of these lymphocytes before infection. Indeed, a fraction of LACK-specific CD4+ T cells expressed a typical CD62 ligandlowCD44highCD45RBlow phenotype in uninfected mice. LACK-specific T cells were primed in gut-associated lymphoid tissues by cross-reactive microbial Ags as demonstrated by their reactivity with bacterial extracts and by the ability of APCs from the mesenteric LN of BALB/c mice to induce their proliferation. Also, mice in which the digestive tract has been decontaminated exhibited a reduced proportion of LACK-specific T cells expressing a memory/effector phenotype and did not exhibit the early accumulation of IL-4 transcripts induced by L. major. Thus, LACK-specific T cells represent a subset of CD4+ T cells which have acquired the ability to rapidly secrete IL-4 as the result of their priming by cross-reactive microbial Ags. Tracking the fate of these cells may provide information about the regulation of cell-mediated immune responses to gut Ags in physiological and pathological situations. |
| Databáze: | OpenAIRE |
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