siRNA targeted against matrix metalloproteinase 11 inhibits the metastatic capability of murine hepatocarcinoma cell Hca-F to lymph nodes
| Autor: | Wei Wei, Jun Cao, Li Jia, Huimin Zhou, Jianing Zhang, Shujing Wang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Small interfering RNA
Carcinoma Hepatocellular Cell Apoptosis Biology Matrix Metalloproteinase Inhibitors Biochemistry Metastasis Mice RNA interference Cell Movement Matrix Metalloproteinase 11 Cell Line Tumor Lymph node stromal cell medicine Cell Adhesion Gene silencing Animals Gene Silencing RNA Messenger Neoplasm Metastasis RNA Small Interfering Cell Proliferation Liver Neoplasms Cell Biology medicine.disease Molecular biology Immunohistochemistry Gene Expression Regulation Neoplastic medicine.anatomical_structure Lymphatic system Tumor progression Cancer research Lymph Nodes |
| Zdroj: | The international journal of biochemistrycell biology. 39(11) |
| ISSN: | 1357-2725 |
| Popis: | Matrix metalloproteinase-11 (MMP-11) belongs to the particular member of MMP family, a group of zinc-dependent endopeptidases involved in tumor progression, invasion and metastasis. MMP-11 is strongly expressed in tumor cells and stromal fibroblasts located in the immediate vicinity of tumor. This study investigated the possible role of MMP-11 expression in mouse hepatocarcinoma cell line Hca-F with highly lymphatic metastasis potential by RNA interference (RNAi) approach. The results showed that a small interfering RNA (siRNA) targeted against MMP-11 significantly impeded Hca-F cells proliferation and colony formation in soft agar, as well as resulted in Hca-F cell apoptosis. This reduction of MMP-11 expression also led to the decreased migration and adhesion of Hca-F cells dramatically both in vitro and in vivo. Furthermore, in vivo metastasis assay indicated that down-regulation of MMP-11 expression in Hca-F cells attenuated the metastatic potential of Hca-F cells to peripheral lymph nodes. These data together provide compelling evidence into the function of MMP-11 and suggest that MMP-11 act as a tumor lymphatic metastasis-associated gene, and could represent a new potential target for gene therapy. |
| Databáze: | OpenAIRE |
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