On the Regenerative Capacity of Human Skeletal Muscle
| Autor: | Ulrich Knauf, Rustam R. Mundegar, Stefan Zimmermann, Anton Wernig, Uwe M. Martens, Oliver Högemeier, Margit Zweyer, Ralf Schäfer |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Senescence Telomerase Adolescent Population Biomedical Engineering Medicine (miscellaneous) Enzyme-Linked Immunosorbent Assay Bioengineering Biology Biomaterials medicine Humans Regeneration Myocyte Child Muscle Skeletal education Cells Cultured Cellular Senescence Aged Genetics education.field_of_study Infant Skeletal muscle General Medicine Middle Aged Telomere Immunohistochemistry Cell biology medicine.anatomical_structure Child Preschool Stem cell Cell aging |
| Zdroj: | Artificial Organs. 29:192-198 |
| ISSN: | 1525-1594 0160-564X |
| DOI: | 10.1111/j.1525-1594.2005.29033.x |
| Popis: | The proliferative capacity of organotypic muscle stem cells, the satellite cells, from nine healthy human donors aged between 2 and 78 years was investigated. There was a loss in proliferative capacity with age, but the oldest donors (76, 78 years) would still be able to replace their musculature several times. Depending on frequency of desmin-positive (i.e., myogenic) cells during prolonged expansion, myoblast cultures could be designated as stable or unstable. There was a weak correlation between mean telomere lengths (estimated by flow-FISH) and remaining mean population doublings until senescence. A bimodal distribution of mean telomere lengths was apparent in both stable and unstable myoblast cultures and could be assigned to populations of differently sized cells. Furthermore, due to the presence of nonmyogenic cells with longer telomeres, unstable cultures tended to show an increasing rather than decreasing mean telomeric length on expansion. Bimodal distributions in myoblast cultures could be due to hitherto undefined myoblast populations. |
| Databáze: | OpenAIRE |
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