Complete Protection against Influenza Virus H1N1 Strain A/PR/8/34 Challenge in Mice Immunized with Non-Adjuvanted Novirhabdovirus Vaccines
| Autor: | Emilie Mérour, Ronan N. Rouxel, Stéphane Biacchesi, Michel Brémont |
|---|---|
| Přispěvatelé: | Unité de recherche Virologie et Immunologie Moléculaires (VIM), Institut National de la Recherche Agronomique (INRA), ABIVAX France, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Moriya Tsuji, Bremont-Deletang, Michel |
| Rok vydání: | 2016 |
| Předmět: |
RNA viruses
0301 basic medicine Influenza Viruses Viral Diseases Physiology [SDV]Life Sciences [q-bio] viruses lcsh:Medicine Pathology and Laboratory Medicine medicine.disease_cause Biochemistry Madin Darby Canine Kidney Cells Mice Immunologic Adjuvants Influenza A Virus H1N1 Subtype Immune Physiology Medicine and Health Sciences Influenza A virus Public and Occupational Health Fluorescent Antibody Technique Indirect lcsh:Science Mononegavirales Mice Inbred BALB C Vaccines Synthetic Vaccines Novirhabdovirus Immune System Proteins Multidisciplinary biology Viral Vaccine Vaccination Animal Models Vaccination and Immunization Recombinant Proteins 3. Good health Hemagglutinins Infectious Diseases Medical Microbiology Viral Pathogens Viruses Female Pathogens Plasmids Research Article Carps Infectious hematopoietic necrosis virus Immunology Hemagglutinin (influenza) Mouse Models Research and Analysis Methods Microbiology Antibodies Virus Cell Line 03 medical and health sciences Dogs Model Organisms Orthomyxoviridae Infections medicine Animals Antigens Microbial Pathogens Biology and life sciences lcsh:R Organisms Proteins biology.organism_classification Antibodies Neutralizing Virology Influenza Microscopy Electron 030104 developmental biology 13. Climate action biology.protein lcsh:Q Viral hemorrhagic septicemia Preventive Medicine Orthomyxoviruses |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2016, 11 (10), pp.1-17. ⟨10.1371/journal.pone.0164245⟩ Plos One 10 (11), 1-17. (2016) PLoS ONE, Vol 11, Iss 10, p e0164245 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Novirhabdoviruses like Viral Hemorrhagic Septicemia Virus (VHSV) and Infectious Hematopoietic Necrosis Virus (IHNV) are fish-infecting Rhabdoviruses belonging to the Mononegavirales order. By reverse genetics, we previously showed that a recombinant VHSV expressing the West Nile Virus (WNV) E glycoprotein could serve as a vaccine platform against WNV. In the current study, we aimed to evaluate the potential of the Novirhabdovirus platform as a vaccine against influenza virus. Recombinant Novirhabdoviruses, rVHSV-HA and rIHNV-HA, expressing at the viral surface the hemagglutinin HA ectodomain were generated and used to immunized mice. We showed that mice immunized with either, rVHSV-HA or rIHNV-HA, elicited a strong neutralizing antibody response against influenza virus. A complete protection was conferred to the immunized mice when challenged with a lethal dose of influenza H1N1 A/PR/8/34 virus. Furthermore we showed that although acting as inert antigen in mice, since naturally inactivated over 20 degrees C, mice immunized with rVHSV-HA or rIHNV-HA in the absence of adjuvant were also completely protected from a lethal challenge. Novirhabdoviruses platform are of particular interest as vaccines for mammals since they are cost effective to produce, relatively easy to generate and very effective to protect immunized animals. |
| Databáze: | OpenAIRE |
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