Molecular Characterization of Terpenoid Biosynthetic Genes and Terpenoid Accumulation in Phlomis umbrosa Turczaninow
| Autor: | Yeon Bok Kim, Young-Seob Lee, Sanghoon Lee, Sang Un Park, Sin-Hee Han, Chinreddy Subramanyam Reddy, Si-Yoon Hwang, Beom-Heon Song |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
South asia Iridoid medicine.drug_class Plant Science Horticulture Biology lcsh:Plant culture 01 natural sciences 03 medical and health sciences medicine Phlomis umbrosa lcsh:SB1-1110 Sesamoside Gene 030304 developmental biology chemistry.chemical_classification 0303 health sciences Glycoside Terpenoid transcript level chemistry Biochemistry terpenoid 010606 plant biology & botany Biosynthetic genes |
| Zdroj: | Horticulturae, Vol 6, Iss 76, p 76 (2020) Horticulturae Volume 6 Issue 4 |
| ISSN: | 2311-7524 |
| Popis: | The root of Phlomis umbrosa has traditionally been used as a medicine in South Asian nations to treat colds and bone fractures, to staunch bleeding, and as an anti-inflammatory, and such use continues today. We identified 10 genes that are involved in terpenoid biosynthesis, while using the Illumina/Solexa HiSeq2000 platform. We investigated the transcript levels of the 10 genes using quantitative real-time PCR and quantified the level of terpenoid accumulation in different organs of P. umbrosa while using high-performance liquid chromatography. The transcript levels of PuHDR and PuHMGR1 were the highest among the studied genes. Sesamoside, an iridoid glycoside, appeared in higher quantity than shanzhiside methylester, umbroside (8-O-acetyl shanzhiside methyl ester), and acteoside. We speculate that PuHDR and PuHMGR1 may contribute to terpenoid biosynthesis in P. umbrosa. This study highlights the molecular mechanisms that underlie iridoid glycoside biosynthesis in P. umbrosa. |
| Databáze: | OpenAIRE |
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